ArticleProceedings of the National Academy of Sciences of the United States of America2013
Actin cytoskeleton of chemotactic amoebae operates close to the onset of oscillations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 69 citations in OpenAlex.
- Anticipation of periodic events influences cell motility in amoeba proteus.Scientific reports · 2026Article
- Article
- Long-term quantification and brief-pulse optogenetic perturbation of nucleocytoplasmic GtaC dynamics during Dictyostelium development.microPublication biology · 2026Article
- Optogenetic control of cAMP oscillations reveals frequency-selective transcription factor dynamics in Dictyostelium.Development (Cambridge, England) · 2025Article
- Energy partitioning in the cell cortex.Nature physics · 2024Article
- Active oscillations in microscale navigation.Animal cognition · 2023Review
- The excitable nature of polymerizing actin and the Belousov-Zhabotinsky reaction.Frontiers in cell and developmental biology · 2023Article
- Intermittent F-actin Perturbations by Magnetic Fields Inhibit Breast Cancer Metastasis.Research (Washington, D.C.) · 2023Article
- Intermittent ERK oscillations downstream of FGF in mouse embryonic stem cells.Development (Cambridge, England) · 2022Article
- Excitable actin dynamics and amoeboid cell migration.PloS one · 2021Article
- Screening by changes in stereotypical behavior during cell motility.Scientific reports · 2019Article
- Rhythmicity and waves in the cortex of single cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- A sharp-edge-based acoustofluidic chemical signal generator.Lab on a chip · 2018Article
- Modeling random crawling, membrane deformation and intracellular polarity of motile amoeboid cells.PloS one · 2018Article
- The threshold of an excitable system serves as a control mechanism for noise filtering during chemotaxis.PloS one · 2018Article
- Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation.Journal of visualized experiments : JoVE · 2017Article
- Article
- Frequency and amplitude control of cortical oscillations by phosphoinositide waves.Nature chemical biology · 2016Article
- Gβ Regulates Coupling between Actin Oscillators for Cell Polarity and Directional Migration.PLoS biology · 2016Article
- Feedback mechanisms in a mechanical model of cell polarization.Physical biology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rapid reorganization of the actin cytoskeleton in response to external stimuli is an essential property of many motile eukaryotic cells. Here, we report evidence that the actin machinery of chemotactic Dictyostelium cells operates close to an oscillatory instability. When averaging the actin response of many cells to a short pulse of the chemoattractant cAMP, we observed a transient accumulation of cortical actin reminiscent of a damped oscillation. At the single-cell level, however, the response dynamics ranged from short, strongly damped responses to slowly decaying, weakly damped oscillations. Furthermore, in a small subpopulation, we observed self-sustained oscillations in the cortical F-actin concentration. To substantiate that an oscillatory mechanism governs the actin dynamics in these cells, we systematically exposed a large number of cells to periodic pulse trains of different frequencies. Our results indicate a resonance peak at a stimulation period of around 20 s. We propose a delayed feedback model that explains our experimental findings based on a time-delay in the regulatory network of the actin system. To test the model, we performed stimulation experiments with cells that express GFP-tagged fusion proteins of Coronin and actin-interacting protein 1, as well as knockout mutants that lack Coronin and actin-interacting protein 1. These actin-binding proteins enhance the disassembly of actin filaments and thus allow us to estimate the delay time in the regulatory feedback loop. Based on this independent estimate, our model predicts an intrinsic period of 20 s, which agrees with the resonance observed in our periodic stimulation experiments.
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