ArticleProceedings of the National Academy of Sciences of the United States of America2013
Blastocyst complementation generates exogenic pancreas in vivo in apancreatic cloned pigs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 129 papers.
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Who cites it
129 citing papers in PubMed.
- The making of cells and organs in xenogeneic animal bioreactor.Signal transduction and targeted therapy · 2026Review
- Generation of exogenous kidneys via CRISPR/Cas9 mediated blastocyst complementation targeting Osr1 gene in mice.Scientific reports · 2025Article
- Will Human-Animal Chimeras Cause Moral Confusion? Exploring Public Attitudes.Journal of bioethical inquiry · 2025Article
- Generation of parathyroid glands from pluripotent stem cells.Endocrine journal · 2025Review
- Gastruloid patterning reflects division of labor among biased stem cell clones.bioRxiv : the preprint server for biology · 2025Article
- Blastocyst complementation: current progress and future directions in xenogeneic organogenesis.Stem cell research & therapy · 2025Review
- Review
- Current Techniques of Gene Editing in Pigs for Xenotransplantation.Transplant international : official journal of the European Society for Organ Transplantation · 2025Review
- Generation of allogenic chimera carrying mutations in PDX1 and TP53 genes via phytohemagglutinin-mediated blastomere aggregation in pigs.In vitro cellular & developmental biology. Animal · 2024Article
- Skin graft with dermis and appendages generated in vivo by cell competition.Nature communications · 2024Article
- Interspecies chimerism with human embryonic stem cells generates functional human dopamine neurons at low efficiency.Stem cell reports · 2024Article
- Functional calcium-responsive parathyroid glands generated using single-step blastocyst complementation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Animal Disease Models and Patient-iPS-Cell-Derived In Vitro Disease Models for Cardiovascular Biology-How Close to Disease?Biology · 2023Review
- Development of a Method for the In Vivo Generation of Allogeneic Hearts in Chimeric Mouse Embryos.International journal of molecular sciences · 2023Article
- Review
- Towards human organ generation using interspecies blastocyst complementation: Challenges and perspectives for therapy.Frontiers in cell and developmental biology · 2023Review
- Transplantation of human cells into Interleukin-2 receptor gamma gene knockout pigs under several conditions.Regenerative therapy · 2022Article
- Article
- From a Single Cell to a Whole Human Liver: Disease Modeling and Transplantation.Seminars in liver disease · 2022Article
- Article
69 more citing papers are in PubMed but not listed here.
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11 authors.
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No grant is acknowledged in the PubMed record.
Abstract
In the field of regenerative medicine, one of the ultimate goals is to generate functioning organs from pluripotent cells, such as ES cells or induced pluripotent stem cells (PSCs). We have recently generated functional pancreas and kidney from PSCs in pancreatogenesis- or nephrogenesis-disabled mice, providing proof of principle for organogenesis from PSCs in an embryo unable to form a specific organ. Key when applying the principles of in vivo generation to human organs is compensation for an empty developmental niche in large nonrodent mammals. Here, we show that the blastocyst complementation system can be applied in the pig using somatic cell cloning technology. Transgenic approaches permitted generation of porcine somatic cell cloned embryos with an apancreatic phenotype. Complementation of these embryos with allogenic blastomeres then created functioning pancreata in the vacant niches. These results clearly indicate that a missing organ can be generated from exogenous cells when functionally normal pluripotent cells chimerize a cloned dysorganogenetic embryo. The feasibility of blastocyst complementation using cloned porcine embryos allows experimentation toward the in vivo generation of functional organs from xenogenic PSCs in large animals.
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