ArticleThe Journal of biological chemistry2013
Three-dimensional structure of Saccharomyces invertase: role of a non-catalytic domain in oligomerization and substrate specificity.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 106 citations in OpenAlex.
- Synergistic Orchestration of Chlorogenic Acid Analogs in Sugarcane Molasses Polyphenols for Sucrase Inhibition: Insights into a Multilevel Inhibitory Mechanism.Foods (Basel, Switzerland) · 2026Article
- Evolutionary diversification of invertase paralogs couples carbon metabolism and sexual reproduction in fission yeasts.Molecular biology and evolution · 2026Article
- Kombucha-Derived Cellulose Non-wovens: Growth Optimization, Mechanics, and Recycling.bioRxiv : the preprint server for biology · 2026Article
- Enhanced fructooligosaccharides synthesis by engineered Trichoderma atroviride β-fructofuranosidase.Applied microbiology and biotechnology · 2026Article
- Improving the hydrophilic microenvironment surrounding the catalytic site of fructosyltransferase enhances its catalytic ability.Biotechnology letters · 2025Article
- Educational activity of enzyme kinetics in an undergraduate biochemistry course: invertase enzyme as a model.Journal of microbiology & biology education · 2024Article
- Frequent Acquisition of Glycoside Hydrolase Family 32 (GH32) Genes from Bacteria via Horizontal Gene Transfer Drives Adaptation of Invertebrates to Diverse Sources of Food and Living Habitats.International journal of molecular sciences · 2024Article
- Oligomeric Structure of Yeast and Other Invertases Governs Specificity.Sub-cellular biochemistry · 2024Review
- Highly complete long-read genomes reveal pangenomic variation underlying yeast phenotypic diversity.Genome research · 2023Article
- Protein engineering of invertase for enhancing yeast dough fermentation under high-sucrose conditions.Folia microbiologica · 2023Article
- Safety evaluation of the food enzyme β-fructofuranosidase from the non-genetically modifiedEFSA journal. European Food Safety Authority · 2023Article
- Molecular Level Sucrose Quantification: A Critical Review.Sensors (Basel, Switzerland) · 2022Review
- Insights into the Structure of the Highly Glycosylated Ffase fromInternational journal of molecular sciences · 2022Article
- Expression of an extremophilic xylanase in Nicotiana benthamiana and its use for the production of prebiotic xylooligosaccharides.Scientific reports · 2022Article
- Article
- Enzymatic and structural characterization of β-fructofuranosidase from the honeybee gut bacterium Frischella perrara.Applied microbiology and biotechnology · 2022Article
- Enzyme-functionalised, core/shell magnetic nanoparticles for selective pH-triggered sucrose capture.RSC advances · 2021Article
- Exploring the Molecular Basis for Substrate Affinity and Structural Stability in Bacterial GH39 β-Xylosidases.Frontiers in bioengineering and biotechnology · 2020Article
- Evolution of metabolic novelty: A trichome-expressed invertase creates specialized metabolic diversity in wild tomato.Science advances · 2019Article
- UniqueJournal of biological engineering · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Invertase is an enzyme that is widely distributed among plants and microorganisms and that catalyzes the hydrolysis of the disaccharide sucrose into glucose and fructose. Despite the important physiological role of Saccharomyces invertase (SInv) and the historical relevance of this enzyme as a model in early biochemical studies, its structure had not yet been solved. We report here the crystal structure of recombinant SInv at 3.3 Å resolution showing that the enzyme folds into the catalytic β-propeller and β-sandwich domains characteristic of GH32 enzymes. However, SInv displays an unusual quaternary structure. Monomers associate in two different kinds of dimers, which are in turn assembled into an octamer, best described as a tetramer of dimers. Dimerization plays a determinant role in substrate specificity because this assembly sets steric constraints that limit the access to the active site of oligosaccharides of more than four units. Comparative analysis of GH32 enzymes showed that formation of the SInv octamer occurs through a β-sheet extension that seems unique to this enzyme. Interaction between dimers is determined by a short amino acid sequence at the beginning of the β-sandwich domain. Our results highlight the role of the non-catalytic domain in fine-tuning substrate specificity and thus supplement our knowledge of the activity of this important family of enzymes. In turn, this gives a deeper insight into the structural features that rule modularity and protein-carbohydrate recognition.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.