ArticleCellular & molecular biology letters2013
GABA exists as a negative regulator of cell proliferation in spermatogonial stem cells. [corrected].
Article in Cellular & molecular biology letters, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Different sex determination systems in two closely related Eurasian minnow (Phoxinus) species.Heredity · 2026Article
- Gonadotropins differentially regulate testicular cell adhesion and junctional complexes during flatfish spermiogenesis through the oxytocin and relaxin signaling pathways.Frontiers in cell and developmental biology · 2025Article
- Toxic Effects of Methamphetamine on Reproductive Systems, Embryo Development, and Newborns: An Evidence-based Review.Current medicinal chemistry · 2025Review
- Alginate oligosaccharide extends the service lifespan by improving the sperm metabolome and gut microbiota in an aging Duroc boars model.Frontiers in cellular and infection microbiology · 2023Article
- Finding Solutions for Fibrosis: Understanding the Innate Mechanisms Used by Super-Regenerator Vertebrates to Combat Scarring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Constitutive activation of CTNNB1 results in a loss of spermatogonial stem cell activity in mice.PloS one · 2021Article
- Network Analyses Predict Small RNAs That Might Modulate Gene Expression in the Testis and Epididymis ofFrontiers in genetics · 2021Article
- GABA inhibits proliferation and self-renewal of mouse retinal progenitor cell.Cell death discovery · 2019Article
- GABAergic Alterations in the Rat Testis after Methamphetamine Exposure.International journal of medical sciences · 2018Article
- Unraveling transcriptome dynamics in human spermatogenesis.Development (Cambridge, England) · 2017Article
- Molecular bioelectricity: how endogenous voltage potentials control cell behavior and instruct pattern regulation in vivo.Molecular biology of the cell · 2014Article
- Reprogramming cells and tissue patterning via bioelectrical pathways: molecular mechanisms and biomedical opportunities.Wiley interdisciplinary reviews. Systems biology and medicineReview
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
γ-amino butyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA is also found in many peripheral tissues, where it has important functions during development. Here, we identified the existence of the GABA system in spermatogonial stem cells (SSCs) and found that GABA negatively regulates SSC proliferation. First, we demonstrated that GABA and its synthesizing enzymes were abundant in the testes 6 days postpartum (dpp), suggesting that GABA signaling regulates SSCs function in vivo. In order to directly examine the effect of GABA on SSC proliferation, we then established an in vitro culture system for long-term expansion of SSCs. We showed that GABAA receptor subunits, including α1, α5, β1, β2, β3 and γ3, the synthesizing enzyme GAD67, and the transporter GAT-1, are expressed in SSCs. Using phosphorylated histone H3 (pH3) staining, we demonstrated that GABA or the GABAAR-specific agonist muscimol reduced the proliferation of SSCs. This GABA regulation of SSC proliferation was shown to be independent of apoptosis using the TUNEL assay. These results suggest that GABA acts as a negative regulator of SSC proliferation to maintain the homeostasis of spermatogenesis in the testes.
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