ArticleNucleic acids research2013
The σ70 region 1.2 regulates promoter escape by unwinding DNA downstream of the transcription start site.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- The modular architecture of sigma factors in cyanobacteria: a framework to assess their diversity and understand their evolution.BMC genomics · 2024Article
- Ureidothiophene inhibits interaction of bacterial RNA polymerase with -10 promotor element.Nucleic acids research · 2020Article
- Inhibition of RNA Polymerase by Rifampicin and Rifamycin-Like Molecules.EcoSal Plus · 2020Article
- Compensating the Fitness Costs of Synonymous Mutations.Molecular biology and evolution · 2016Article
- Region 4 of Rhizobium etli Primary Sigma Factor (SigA) Confers Transcriptional Laxity in Escherichia coli.Frontiers in microbiology · 2016Article
- An aromatic residue switch in enhancer-dependent bacterial RNA polymerase controls transcription intermediate complex activity.Nucleic acids research · 2013Article
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Authors and funding
2 authors.
Funding
Abstract
The mechanisms of abortive synthesis and promoter escape during initiation of transcription are poorly understood. Here, we show that, after initiation of RNA synthesis, non-specific interaction of σ(70) region 1.2, present in all σ(70) family factors, with the non-template strand around position -4 relative to the transcription start site facilitates unwinding of the DNA duplex downstream of the transcription start site. This leads to stabilization of short RNA products and allows their extension, i.e. promoter escape. We show that this activity of σ(70) region 1.2 is assisted by the β-lobe domain, but does not involve the β'-rudder or the β'-switch-2, earlier proposed to participate in promoter escape. DNA sequence independence of this function of σ(70) region 1.2 suggests that it may be conserved in all σ(70) family factors. Our results indicate that the abortive nature of initial synthesis is caused, at least in part, by failure to open the downstream DNA by the β-lobe and σ region 1.2.
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