ArticleInternational journal of nanomedicine2013
Intracellular distribution of nontargeted quantum dots after natural uptake and microinjection.
Article in International journal of nanomedicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Bridging Smart Nanosystems with Clinically Relevant Models and Advanced Imaging for Precision Drug Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- NIR fluorescence imaging and treatment for cancer immunotherapy.Journal for immunotherapy of cancer · 2022Review
- Intracellular Trafficking and Distribution of Cd and InP Quantum Dots in HeLa and ML-1 Thyroid Cancer Cells.Nanomaterials (Basel, Switzerland) · 2022Article
- Article
- Comparative In Vitro Cytotoxicity Study of Carbon Dot-Based Organometallic Nanoconjugates: Exploration of Their Cell Proliferation, Uptake, and Localization in Cancerous and Normal Cells.Oxidative medicine and cellular longevity · 2022Article
- Polymer-based nanoparticles: fabrication to applications-the many faces of DC8,9PC and albumin.Biophysical reviews · 2021Review
- Immune cell labelling and tracking: implications for adoptive cell transfer therapies.EJNMMI radiopharmacy and chemistry · 2021Review
- Imaging techniques in nanomedical research.European journal of histochemistry : EJH · 2020Article
- Probing Intracellular Dynamics Using Fluorescent Carbon Dots Produced by Femtosecond LaserACS omega · 2020Article
- pH-triggered endosomal escape of pore-forming Listeriolysin O toxin-coated gold nanoparticles.Journal of nanobiotechnology · 2019Article
- Study on intracellular delivery of liposome encapsulated quantum dots using advanced fluorescence microscopy.Scientific reports · 2019Article
- Uptake and distribution of carboxylated quantum dots in human mesenchymal stem cells: cell growing density matters.Journal of nanobiotechnology · 2019Article
- Impact of Quantum Dot Surface on Complex Formation with Chlorin e₆ and Photodynamic Therapy.Nanomaterials (Basel, Switzerland) · 2018Article
- Fluorescent ZnS Quantum Dots-Phosphoethanolamine Nanoconjugates for Bioimaging Live Cells in Cancer Research.ACS omega · 2018Article
- Efficient Delivery of Quantum Dots into the Cytosol of Cells Using Cell-Penetrating Poly(disulfide)s.Journal of the American Chemical Society · 2017Article
- One-Pot Aqueous Synthesis of Fluorescent Ag-In-Zn-S Quantum Dot/Polymer Bioconjugates for Multiplex Optical Bioimaging of Glioblastoma Cells.Contrast media & molecular imaging · 2017Article
- Skin-derived mesenchymal stem cells as quantum dot vehicles to tumors.International journal of nanomedicine · 2017Article
- Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery.Beilstein journal of nanotechnology · 2017Article
- Penetration of CdSe/ZnS quantum dots into differentiated vs undifferentiated Caco-2 cells.Journal of nanobiotechnology · 2016Article
- Accumulation and Toxicity of Superparamagnetic Iron Oxide Nanoparticles in Cells and Experimental Animals.International journal of molecular sciences · 2016Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe purpose of this study was to elucidate the mechanism of natural uptake of nonfunctionalized quantum dots in comparison with microinjected quantum dots by focusing on their time-dependent accumulation and intracellular localization in different cell lines.
methodsThe accumulation dynamics of nontargeted CdSe/ZnS carboxyl-coated quantum dots (emission peak 625 nm) was analyzed in NIH3T3, MCF-7, and HepG2 cells by applying the methods of confocal and steady-state fluorescence spectroscopy. Intracellular colocalization of the quantum dots was investigated by staining with Lysotracker(®).
resultsThe uptake of quantum dots into cells was dramatically reduced at a low temperature (4 °C), indicating that the process is energy-dependent. The uptake kinetics and imaging of intracellular localization of quantum dots revealed three accumulation stages of carboxyl-coated quantum dots at 37 °C, ie, a plateau stage, growth stage, and a saturation stage, which comprised four morphological phases: adherence to the cell membrane; formation of granulated clusters spread throughout the cytoplasm; localization of granulated clusters in the perinuclear region; and formation of multivesicular body-like structures and their redistribution in the cytoplasm. Diverse quantum dots containing intracellular vesicles in the range of approximately 0.5-8 μm in diameter were observed in the cytoplasm, but none were found in the nucleus. Vesicles containing quantum dots formed multivesicular body-like structures in NIH3T3 cells after 24 hours of incubation, which were Lysotracker-negative in serum-free medium and Lysotracker-positive in complete medium. The microinjected quantum dots remained uniformly distributed in the cytosol for at least 24 hours.
conclusionNatural uptake of quantum dots in cells occurs through three accumulation stages via a mechanism requiring energy. The sharp contrast of the intracellular distribution after microinjection of quantum dots in comparison with incubation as well as the limited transfer of quantum dots from vesicles into the cytosol and vice versa support the endocytotic origin of the natural uptake of quantum dots. Quantum dots with proteins adsorbed from the culture medium had a different fate in the final stage of accumulation from that of the protein-free quantum dots, implying different internalization pathways.
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