ArticleMolecular & cellular proteomics : MCP2013
Structurally informative tandem mass spectrometry of highly sulfated natural and chemoenzymatically synthesized heparin and heparan sulfate glycosaminoglycans.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 42 citations in OpenAlex.
- Investigation of the Experimental Parameters of Ultraviolet Photodissociation for the Structural Characterization of Chondroitin Sulfate Glycosaminoglycan Isomers.Journal of the American Society for Mass Spectrometry · 2021Article
- HepParser: An Intelligent Software Program for Deciphering Low-Molecular-Weight Heparin Based on Mass Spectrometry.Frontiers in chemistry · 2021Article
- Developments in Mass Spectrometry for Glycosaminoglycan Analysis: A Review.Molecular & cellular proteomics : MCP · 2021Review
- Structural Characterization of Glycosaminoglycan Carbohydrates Using Ultraviolet Photodissociation.Analytical chemistry · 2019Article
- Analysis of sulfates on low molecular weight heparin using mass spectrometry: structural characterization of enoxaparin.Expert review of proteomics · 2018Review
- Structural elucidation of fucosylated chondroitin sulfates from sea cucumber using FTICR-MS/MS.European journal of mass spectrometry (Chichester, England) · 2018Article
- Negative Electron Transfer Dissociation Sequencing of Increasingly Sulfated Glycosaminoglycan Oligosaccharides on an Orbitrap Mass Spectrometer.Journal of the American Society for Mass Spectrometry · 2017Article
- Single Stage Tandem Mass Spectrometry Assignment of the C-5 Uronic Acid Stereochemistry in Heparan Sulfate Tetrasaccharides using Electron Detachment Dissociation.Journal of the American Society for Mass Spectrometry · 2017Article
- Structural Analysis of Heparin-Derived 3-O-Sulfated Tetrasaccharides: Antithrombin Binding Site Variants.Journal of pharmaceutical sciences · 2017Article
- Vibrational Signatures of Isomeric Lithiated N-acetyl-D-hexosamines by Gas-Phase Infrared Multiple-Photon Dissociation (IRMPD) Spectroscopy.Journal of the American Society for Mass Spectrometry · 2017Article
- Characterization of Glycan Structures of Chondroitin Sulfate-Glycopeptides Facilitated by Sodium Ion-Pairing and Positive Mode LC-MS/MS.Journal of the American Society for Mass Spectrometry · 2017Article
- Assignment Of Hexuronic Acid Stereochemistry In Synthetic Heparan Sulfate Tetrasaccharides With 2-International journal of mass spectrometry · 2015Article
- The Interaction of Heparin Tetrasaccharides with Chemokine CCL5 Is Modulated by Sulfation Pattern and pH.The Journal of biological chemistry · 2015Article
- Linkage and anomeric differentiation in trisaccharides by sequential fragmentation and variable-wavelength infrared photodissociation.Journal of the American Society for Mass Spectrometry · 2015Article
- Differentiating chondroitin sulfate glycosaminoglycans using collision-induced dissociation; uronic acid cross-ring diagnostic fragments in a single stage of tandem mass spectrometry.European journal of mass spectrometry (Chichester, England) · 2015Article
- Heparan sulfate 3-O-sulfation: a rare modification in search of a function.Matrix biology : journal of the International Society for Matrix Biology · 2014Review
- High-field asymmetric-waveform ion mobility spectrometry and electron detachment dissociation of isobaric mixtures of glycosaminoglycans.Journal of the American Society for Mass Spectrometry · 2014Article
- Oligosaccharide analysis by mass spectrometry: a review of recent developments.Analytical chemistry · 2014Review
- De novo sequencing of heparan sulfate oligosaccharides by electron-activated dissociation.Analytical chemistry · 2013Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The highly sulfated glycosaminoglycan oligosaccharides derived from heparin and heparan sulfate have been a highly intractable class of molecules to analyze by tandem mass spectrometry. Under the many methods of ion activation, this class of molecules generally exhibits SO3 loss as the most significant fragmentation pathway, interfering with the assignment of the location of sulfo groups in glycosaminoglycan chains. We report here a method that stabilizes sulfo groups and facilitates the complete structural analysis of densely sulfated (two or more sulfo groups per disaccharide repeat unit) heparin and heparan sulfate oligomers. This is achieved by complete removal of all ionizable protons, either by charging during electrospray ionization or by Na(+)/H(+) exchange. The addition of millimolar levels of NaOH to the sample solution facilitates the production of precursor ions that meet this criterion. This approach is found to work for a variety of heparin sulfate oligosaccharides derived from natural sources or produced by chemoenzymatic synthesis, with up to 12 saccharide subunits and up to 11 sulfo groups.
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