ArticleSensors (Basel, Switzerland)2013
Detection of Micrococcus luteus biofilm formation in microfluidic environments by pH measurement using an ion-sensitive field-effect transistor.
Article in Sensors (Basel, Switzerland), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Epinephrine Affects Ribosomes, Cell Division, and Catabolic Processes inMicroorganisms · 2023Article
- MALDI-TOF as a tool for microbiological monitoring in areas considered aseptic.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Cultivation-Based Quantification and Identification of Bacteria at Two Hygienic Key Sides of Domestic Washing Machines.Microorganisms · 2021Article
- AC Electric Field Mediated Assembly of Bacterial Tetrads.ACS omega · 2020Article
- Hybrid Silicon Nanowire Devices and Their Functional Diversity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Review
- Smart Cell Culture Systems: Integration of Sensors and Actuators into Microphysiological Systems.ACS chemical biology · 2018Review
- A simple method for decreasing the liquid junction potential in a flow-through-type differential pH sensor probe consisting of pH-FETs by exerting spatiotemporal control of the liquid junction.Sensors (Basel, Switzerland) · 2015Article
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Authors and funding
4 authors.
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Abstract
Biofilm formation in microfluidic channels is difficult to detect because sampling volumes are too small for conventional turbidity measurements. To detect biofilm formation, we used an ion-sensitive field-effect transistor (ISFET) measurement system to measure pH changes in small volumes of bacterial suspension. Cells of Micrococcus luteus (M. luteus) were cultured in polystyrene (PS) microtubes and polymethylmethacrylate (PMMA)-based microfluidic channels laminated with polyvinylidene chloride. In microtubes, concentrations of bacteria and pH in the suspension were analyzed by measuring turbidity and using an ISFET sensor, respectively. In microfluidic channels containing 20 μL of bacterial suspension, we measured pH changes using the ISFET sensor and monitored biofilm formation using a microscope. We detected acidification and alkalinization phases of M. luteus from the ISFET sensor signals in both microtubes and microfluidic channels. In the alkalinization phase, after 2 day culture, dense biofilm formation was observed at the bottom of the microfluidic channels. In this study, we used an ISFET sensor to detect biofilm formation in clinical and industrial microfluidic environments by detecting alkalinization of the culture medium.
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