ArticleJournal of virology2013
Construction and characterization of an infectious molecular clone of Koala retrovirus.
Article in Journal of virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Gammaretrovirus Infections in Humans in the Past, Present, and Future: Have We Defeated the Pathogen?Pathogens (Basel, Switzerland) · 2026Review
- Elevated oncogene expressions in koala infected with multiple koala retrovirus subtypes: a preliminary study.Virus genes · 2025Article
- A recent gibbon ape leukemia virus germline integration in a rodent from New Guinea.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Molecular Diagnosis of Koala Retrovirus (KoRV) in South Australian Koalas (Animals : an open access journal from MDPI · 2021Article
- Koala retrovirus epidemiology, transmission mode, pathogenesis, and host immune response in koalas (Phascolarctos cinereus): a review.Archives of virology · 2020Review
- Helping koalas battle disease - Recent advances in Chlamydia and koala retrovirus (KoRV) disease understanding and treatment in koalas.FEMS microbiology reviews · 2020Review
- Transmission of Koala Retrovirus from Parent Koalas to a Joey in a Japanese Zoo.Journal of virology · 2020Article
- Therapeutic vaccination of koalas harbouring endogenous koala retrovirus (KoRV) improves antibody responses and reduces circulating viral load.NPJ vaccines · 2020Article
- Antibody response against koala retrovirus (KoRV) in koalas harboring KoRV-A in the presence or absence of KoRV-B.Scientific reports · 2019Article
- Review
- Endogenous Gibbon Ape Leukemia Virus Identified in a Rodent (Melomys burtoni subsp.) from Wallacea (Indonesia).Journal of virology · 2016Article
- Episodic Diversifying Selection Shaped the Genomes of Gibbon Ape Leukemia Virus and Related Gammaretroviruses.Journal of virology · 2016Article
- Human and murine APOBEC3s restrict replication of koala retrovirus by different mechanisms.Retrovirology · 2015Article
- Immunomics of the koala (Phascolarctos cinereus).Immunogenetics · 2015Article
- Induction of neutralizing antibodies specific for the envelope proteins of the koala retrovirus by immunization with recombinant proteins or with DNA.Virology journal · 2015Article
- Sequence variation of koala retrovirus transmembrane protein p15E among koalas from different geographic regions.Virology · 2015Article
- Proliferation of endogenous retroviruses in the early stages of a host germ line invasion.Molecular biology and evolution · 2015Article
- A transcriptome resource for the koala (Phascolarctos cinereus): insights into koala retrovirus transcription and sequence diversity.BMC genomics · 2014Article
- Hybridization capture reveals evolution and conservation across the entire Koala retrovirus genome.PloS one · 2014Article
- Koala retroviruses: characterization and impact on the life of koalas.Retrovirology · 2013Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Koala retrovirus (KoRV) is a gammaretrovirus that is currently endogenizing into koalas. Studies on KoRV infection have been hampered by the lack of a replication-competent molecular clone. In this study, we constructed an infectious molecular clone, termed plasmid pKoRV522, of a KoRV isolate (strain Aki) from a koala reared in a Japanese zoo. The virus KoRV522, derived from pKoRV522, grew efficiently in human embryonic kidney (HEK293T) cells, attaining 10(6) focus-forming units/ml. Several mutations in the Gag (L domain) and Env regions reported to be involved in reduction in viral infection/production in vitro are found in pKoRV522, yet KoRV522 replicated well, suggesting that any effects of these mutations are limited. Indeed, a reporter virus pseudotyped with pKoRV522 Env was found to infect human, feline, and mink cell lines efficiently. Analyses of KoRV L-domain mutants showed that an additional PPXY sequence, PPPY, in Gag plays a critical role in KoRV budding. Altogether, our results demonstrate the construction and characterization of the first infectious molecular clone of KoRV. The infectious clone reported here will be useful for elucidating the mechanism of endogenization of the virus in koalas and screening for antiretroviral drugs for KoRV-infected koalas.
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