ArticleJournal of clinical microbiology2013
Optimizing identification of clinically relevant Gram-positive organisms by use of the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Randomized Controlled Trial of Oral Vancomycin Treatment in Clostridioides difficile-Colonized Patients.mSphere · 2021Trial
- Molecular epidemiology of Staphylococcus aureus in households of children with community-associated S aureus skin and soft tissue infections.The Journal of pediatrics · 2014Trial
- Evaluating the Autof MS2600 vs. Biotyper Sirius MALDI-TOF MS: a head-to-head study for routine microbial identification in a university clinical microbiology laboratory.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Human Cellulosimicrobium Infections: A Clinically Oriented Review of Diagnostic and Therapeutic Challenges.Cureus · 2026Review
- Exploring Persistent Apical Periodontitis in Humans: Integrative Genetic, Histological and Microbiological Perspectives for Translational Research.International endodontic journal · 2026Article
- Multidisciplinary Intervention After Dental Trauma in a Young Patient During Sports Practice: A Case Report.Case reports in dentistry · 2026Article
- Experimental study on factors influencing low identification rates and spectral quality ofMicrobiology spectrum · 2025Article
- Metabolites derived from bacterial isolates of the human skin microbiome inhibitMicrobiology spectrum · 2025Article
- Taxonomic Diversity and Antimicrobial Potential of Thermophilic Bacteria from Two Extreme Algerian Hot Springs.Microorganisms · 2025Article
- Isolation and Characterization of Enterocin-ProducingFoods (Basel, Switzerland) · 2025Article
- Rapid Bacterial Identification from Positive Blood Cultures by MALDI-TOF MS Following Short-Term Incubation on Solid Media.Infectious diseases & clinical microbiology · 2024Article
- Evaluation of a Novel Benchtop Tool for Acceleration of Sample Preparation for MALDI-TOF Mass Spectrometry.Journal of clinical microbiology · 2023Article
- Evaluation of the Zybio EXS3000 mass spectrometry in routine identification of Clinical isolates.Heliyon · 2023Article
- Article
- From contamination to infective endocarditis-a population-based retrospective study of Corynebacterium isolated from blood cultures.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Identification performance of MALDI-ToF-MS upon mono- and bi-microbial cultures is cell number and culture proportion dependent.Analytical and bioanalytical chemistry · 2019Article
- Comparison of standard and on-plate extraction protocols for identification of mastitis-causing bacteria by MALDI-TOF MS.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Identification ofJournal of food science and technology · 2018Article
- Safety and Accuracy of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Highly Pathogenic Organisms.Journal of clinical microbiology · 2017Article
- MALDI-TOF mass spectrometry: An emerging tool for unequivocal identification of non-fermenting Gram-negative bacilli.The Indian journal of medical research · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) can be used as a method for the rapid identification of microorganisms. This study evaluated the Bruker Biotyper (MALDI-TOF MS) system for the identification of clinically relevant Gram-positive organisms. We tested 239 aerobic Gram-positive organisms isolated from clinical specimens. We evaluated 4 direct-smear methods, including "heavy" (H) and "light" (L) smears, with and without a 1-μl direct formic acid (FA) overlay. The quality measure assigned to a MALDI-TOF MS identification is a numerical value or "score." We found that a heavy smear with a formic acid overlay (H+FA) produced optimal MALDI-TOF MS identification scores and the highest percentage of correctly identified organisms. Using a score of ≥2.0, we identified 183 of the 239 isolates (76.6%) to the genus level, and of the 181 isolates resolved to the species level, 141 isolates (77.9%) were correctly identified. To maximize the number of correct identifications while minimizing misidentifications, the data were analyzed using a score of ≥1.7 for genus- and species-level identification. Using this score, 220 of the 239 isolates (92.1%) were identified to the genus level, and of the 181 isolates resolved to the species level, 167 isolates (92.2%) could be assigned an accurate species identification. We also evaluated a subset of isolates for preanalytic factors that might influence MALDI-TOF MS identification. Frequent subcultures increased the number of unidentified isolates. Incubation temperatures and subcultures of the media did not alter the rate of identification. These data define the ideal bacterial preparation, identification score, and medium conditions for optimal identification of Gram-positive bacteria by use of MALDI-TOF MS.
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