ArticleJournal of clinical microbiology2013
Optimization of routine identification of clinically relevant Gram-negative bacteria by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and the Bruker Biotyper.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Evaluation of the Zybio EXS3000 mass spectrometry in routine identification of Clinical isolates.Heliyon · 2023Article
- Identification and Subtyping ofMicroorganisms · 2022Article
- Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Analysis for the Identification of Pathogenic Microorganisms: A Review.Microorganisms · 2021Review
- Re-Introduction of Bovine Viral Diarrhea Virus in a Disease-Free Region: Impact on the Affected Cattle Herd and Diagnostic Implications.Pathogens (Basel, Switzerland) · 2021Article
- Evaluation of three sample preparation methods for the identification of clinical strains by using two MALDI-TOF MS systems.Journal of mass spectrometry : JMS · 2021Article
- Current Status of Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology.Molecules (Basel, Switzerland) · 2020Review
- Contamination by Antibiotic-Resistant Bacteria in Selected Environments in Thailand.International journal of environmental research and public health · 2019Article
- Identification and differentiation of avianJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Clinical epidemiology of carbapenem-resistant gram-negative sepsis among hospitalized patients: Shifting burden of disease?American journal of infection control · 2018Article
- Article
- MALDI-TOF mass spectrometry technology for detecting biomarkers of antimicrobial resistance: current achievements and future perspectives.Annals of translational medicine · 2018Review
- Rapid one-step extraction method for the identification of molds using MALDI-TOF MS.Diagnostic microbiology and infectious disease · 2018Article
- Safety and Accuracy of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Highly Pathogenic Organisms.Journal of clinical microbiology · 2017Article
- MALDI-TOF mass spectrometry: An emerging tool for unequivocal identification of non-fermenting Gram-negative bacilli.The Indian journal of medical research · 2017Article
- Culture of Urine Specimens by Use of chromID CPS Elite Medium Can Expedite Escherichia coli Identification and Reduce Hands-On Time in the Clinical Laboratory.Journal of clinical microbiology · 2016Article
- Direct Identification of Aerobic Bacteria by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Is Accurate and Robust.Journal of clinical laboratory analysis · 2016Article
- Plesiomonas shigelloides Revisited.Clinical microbiology reviews · 2016Review
- Methylotroph Infections and Chronic Granulomatous Disease.Emerging infectious diseases · 2016Article
- Reducing time to identification of positive blood cultures with MALDI-TOF MS analysis after a 5-h subculture.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Comparison of chromogenic media for recovery of carbapenemase-producing enterobacteriaceae (CPE) and evaluation of CPE prevalence at a tertiary care academic medical center.Journal of clinical microbiology · 2015Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) might complement and one day replace phenotypic identification of bacteria in the clinical microbiology laboratory, but there is no consensus standard regarding the requirements for its validation prior to clinical use in the United States. The objective of this study was to assess the preanalytical variables influencing Gram-negative identification by use of the Bruker Biotyper MALDI-TOF MS system, including density of organism spotting on a stainless steel target plate and the direct overlay of organisms with formic acid. A heavy smear with formic acid overlay was either superior or equivalent to alternative smear conditions. Microbiological preanalytical variables were also assayed, such as culture medium, growth temperature, and use of serial subculture. Postanalytical analysis included the application of modified species-level identification acceptance criteria. Biotyper identifications were compared with those using traditional phenotypic methods, and discrepancies were resolved with 16S rRNA gene sequencing. Compared to the recommended score cutoffs of the manufacturer, the application of optimized Biotyper score cutoffs for species-level identification increased the rate of identification by 6.75% for the enteric Gram-negative bacteria and 4.25% for the nonfermenting Gram-negative bacteria. Various incubation temperatures, growth medium types, and repeat subcultures did not result in misidentification. We conclude that the Bruker MALDI Biotyper is a robust system for the identification of Gram-negative organisms in the clinical laboratory and that meaningful performance improvements can be made by implementing simple pre- and postanalytical techniques.
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