ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2013
Synaptic vesicle generation from activity-dependent bulk endosomes requires calcium and calcineurin.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 47 citations in OpenAlex.
- Functional Specialization of Clathrin-Independent Endocytic Routes: Mechanisms and Cellular Roles.Traffic (Copenhagen, Denmark) · 2026Review
- Chromatin Packing Domain Engineering Through the Manipulation of Nuclear Cationic States.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Flower Delivery: The Emergence of FLOWER (FWE) as a Multifunctional Regulator of Endo-Lysosome-Related Organelle Trafficking Across Cell Types.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Increased Activity-Dependent Bulk Endocytosis in Huntington's Disease Results From Huntingtin Haploinsufficiency.Journal of neurochemistry · 2025Article
- Piezo1 protects against inflammatory bone loss via a unique CaFrontiers in immunology · 2025Article
- A cellular atlas of calcineurin signaling.Biochimica et biophysica acta. Molecular cell research · 2023Review
- Live-cell imaging of endocytosed synaptophysin around individual hippocampal presynaptic active zones.Frontiers in cellular neuroscience · 2023Article
- Quantum-dot-labeled synuclein seed assay identifies drugs modulating the experimental prion-like transmission.Communications biology · 2022Article
- Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.The EMBO journal · 2022Article
- Imaging Endocytosis Dynamics in Health and Disease.Membranes · 2022Review
- Minibrain kinase and calcineurin coordinate activity-dependent bulk endocytosis through synaptojanin.The Journal of cell biology · 2021Article
- Role of Clathrin and Dynamin in Clathrin Mediated Endocytosis/Synaptic Vesicle Recycling and Implications in Neurological Diseases.Frontiers in cellular neuroscience · 2021Review
- Special Delivery: Potential Mechanisms of Botulinum Neurotoxin Uptake and Trafficking within Motor Nerve Terminals.International journal of molecular sciences · 2020Review
- Endocytosis in the adaptation to cellular stress.Cell stress · 2020Review
- Synaptic vesicle generation from activity-dependent bulk endosomes requires a dephosphorylation-dependent dynamin-syndapin interaction.Journal of neurochemistry · 2019Article
- Loss of myosin Vb promotes apical bulk endocytosis in neonatal enterocytes.The Journal of cell biology · 2019Article
- The Synaptic Vesicle Cycle Revisited: New Insights into the Modes and Mechanisms.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Review
- Activity-dependent bulk endocytosis proteome reveals a key presynaptic role for the monomeric GTPase Rab11.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Impaired Autophagy in Motor Neurons: A Final Common Mechanism of Injury and Death.Physiology (Bethesda, Md.) · 2018Review
- Promotion of endocytosis efficiency through an ATP-independent mechanism at rat calyx of Held terminals.The Journal of physiology · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Activity-dependent bulk endocytosis (ADBE) is the dominant mode of synaptic vesicle (SV) endocytosis during high-frequency stimulation in central nerve terminals. ADBE generates endosomes direct from the plasma membrane, meaning that high concentrations of calcium will be present in their interior due to fluid phase uptake from the extracellular space. Morphological and fluorescent assays were used to track the generation of SVs from bulk endosomes in primary neuronal culture. This process was functionally uncoupled from both SV exocytosis and plasma membrane retrieval events by intervening only after SV fusion and endocytosis were completed. Either intracellular (BAPTA-AM) or intra-endosomal (Rhod-dextran) calcium chelation inhibited SV generation from bulk endosomes, indicating that calcium efflux from this compartment is critical for this process. The V-type ATPase antagonist bafilomycin A1 also arrested SV generation from bulk endosomes, indicating endosomal acidification may be required for calcium efflux. Finally, pharmacological inhibition of the calcium-dependent protein phosphatase calcineurin blocked endosomal SV generation, identifying it as a key downstream effector in this process. These results reveal a novel and key role for the fluid phase uptake of extracellular calcium and its subsequent efflux in the SV lifecycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.