ArticleLipids in health and disease2013
Butylated hydroxytoluene can protect polyunsaturated fatty acids in dried blood spots from degradation for up to 8 weeks at room temperature.
Article in Lipids in health and disease, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Dried Blood Spots to Assess Cardiovascular-Kidney-Metabolic Health.Journal of the American Heart Association · 2025Pooled it
- Feasibility Study of Soybean Oil-Fortified Foods to Alter Blood Content of Linoleic Acid and Body Weight: A Randomized Double-Masked Placebo-Controlled Crossover Trial.The Journal of nutrition · 2026Trial
- Do 6-9-year-old children in Denmark adhere to national dietary recommendations and are there sociodemographic disparities? The Generation Healthy Kids study.European journal of nutrition · 2026Article
- Strategic advances in liposomes technology: translational paradigm in transdermal delivery for skin dermatosis.Journal of nanobiotechnology · 2025Review
- Dried blood spot technique for dietary fat assessment in a human nutritional intervention focused on oily fish intake.Nutrition & metabolism · 2025Article
- The influence of FADS1 and ELOVL2 genetic polymorphisms on polyunsaturated fatty acid composition in response to fish oil supplementation.Lipids in health and disease · 2025Article
- Investigation into temporal changes in the human bloodstain lipidome.International journal of legal medicine · 2025Article
- Approaches for the Inactivation ofApplied biosafety : journal of the American Biological Safety Association · 2024Article
- Dried blood spots in clinical lipidomics: optimization and recent findings.Analytical and bioanalytical chemistry · 2022Review
- OxInflammation at High Altitudes: A Proof of Concept from the Himalayas.Antioxidants (Basel, Switzerland) · 2022Article
- High-Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- DNA methylation of tumour necrosis factor (TNF) alpha gene is associated with specific blood fatty acid levels in a gender-specific manner.Molecular genetics & genomic medicine · 2021Article
- Cell Analysis from Dried Blood Spots: New Opportunities in Immunology, Hematology, and Infectious Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Mass Spectrometric Analysis of Bioactive Sphingolipids in Fungi.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Effects of Dried Blood Spot Storage on Lipidomic Analysis.Molecules (Basel, Switzerland) · 2018Article
- Correlates of whole-blood polyunsaturated fatty acids among young children with moderate acute malnutrition.Nutrition journal · 2017Article
- Development and Validation of an Enzymatic Method for Total Cholesterol Analysis Using Whole Blood Spot.Journal of clinical laboratory analysis · 2016Article
- Short-Term Stability of Whole Blood Polyunsaturated Fatty Acid Content on Filter Paper During Storage at -28 °C.Lipids · 2016Article
- Analysis of breast milk fatty acid composition using dried milk samples.International breastfeeding journal · 2016Article
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Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDried blood spots (DBS) from fingertip prick blood can enable high throughput fatty acid profiling but may be prone to lipid peroxidation during storage. The use of butylated hydroxytoluene (BHT) on chromatography paper can prevent polyunsaturated fatty acid (PUFA) loss but examinations on the length of storage times possible are not comprehensive.
methodIn the first study, venous whole blood was saturated on paper strips pre-soaked with 0, 2.5 or 5.0 mg/mL BHT and exposed to air for up to 28 days. In a second study, the effect of sealing DBS on 5.0 mg/mL BHT-soaked chromatography strips in capped test tubes or vacuum sealed polypropylene bags with and without nitrogen purging was examined over eight weeks. The fatty acid composition of the DBS were determined by gas chromatography and the effect of sample storage on omega-3 biomarkers were examined.
resultsPUFA and omega-3 biomarkers in DBS stored without BHT were dramatically reduced by day 3. In general, BHT delayed decreases in eicosapentaenoic + docosahexaenoic acid from baseline (3.2 ± 0.2 wt%) to 28 days (2.6 ± 0.03 wt%) of storage. In the % n-3 highly unsaturated fatty acids (HUFA) in total HUFA biomarker, BHT was more effective at preventing changes, particularly with 5.0 mg/mL BHT where no differences were detected up to 28 days. Sealed storage with BHT tended to increase the stability of the PUFA in DBS and nitrogen purging did not appear to provide additional benefits. The % n-3 HUFA in total HUFA biomarker also appeared to be more stable in the sealed storage study.
conclusionsThe storage of DBS in sealed containers with BHT may prevent PUFA degradation for up to 8 weeks. The % n-3 HUFA in total HUFA biomarker appears to provide a more consistent assessment of omega-3 status throughout storage as compared with other omega-3 blood biomarkers.
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