ArticleThe New phytologist2013
Prospects and limits of the flow cytometric seed screen--insights from Potentilla sensu lato (Potentilleae, Rosaceae).
Article in The New phytologist, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Development of a low-coverage whole genome sequencing screen for apomixis using a diverse set of Malus germplasm.PLoS genetics · 2026Article
- Apomixis frequency in polyploid wild populations of Psidium cattleyanum f. lucidum (Myrteae, Myrtaceae).Plant reproduction · 2026Article
- Wide diversity in narrow geographic space: genetic, morphological and ploidy variation in three Central EuropeanAoB PLANTS · 2026Article
- Flow Cytometric Seed Screening and Seed Genotyping: Methods for Detecting Reproductive Modes in Flowering Plants.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A novel strategy to study apomixis, automixis, and autogamy in plants.Plant reproduction · 2024Article
- Apomixis and the paradox of sex in plants.Annals of botany · 2024Review
- Reproduction Modes and Conservation Implications in Three PolyploidPlants (Basel, Switzerland) · 2023Article
- Variability of Reproduction Pathways in the Central-European Populations of Hawthorns with Emphasis on Triploids.Plants (Basel, Switzerland) · 2022Article
- Genome Size, Cytotype Diversity and Reproductive Mode Variation ofPlants (Basel, Switzerland) · 2021Article
- The Application of Flow Cytometry for Estimating Genome Size, Ploidy Level Endopolyploidy, and Reproductive Modes in Plants.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Epigenetic Patterns and Geographical Parthenogenesis in the Alpine Plant SpeciesInternational journal of molecular sciences · 2020Article
- Plant apomixis is rare in Himalayan high-alpine flora.Scientific reports · 2019Article
- The morphometrics of autopolyploidy: insignificant differentiation among sexual-apomictic cytotypes.AoB PLANTS · 2019Article
- Sexual intraspecific recombination but notTaxon · 2018Article
- Asymmetric reproductive interference: The consequences of cross-pollination on reproductive success in sexual-apomictic populations ofEcology and evolution · 2018Article
- Pathways to polyploidy: indications of a female triploid bridge in the alpine speciesPlant systematics and evolution = Entwicklungsgeschichte und Systematik der Pflanzen · 2017Article
- Correlations of polyploidy and apomixis with elevation and associated environmental gradients in an alpine plant.AoB PLANTS · 2016Article
- Photoperiod Extension Enhances Sexual Megaspore Formation and Triggers Metabolic Reprogramming in Facultative Apomictic Ranunculus auricomus.Frontiers in plant science · 2016Article
- When sexual meets apomict: genome size, ploidy level and reproductive mode variation of Sorbus aria s.l. and S. austriaca (Rosaceae) in Bosnia and Herzegovina.Annals of botany · 2015Article
- Emergence of apospory and bypass of meiosis via apomixis after sexual hybridisation and polyploidisation.The New phytologist · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The flow cytometric seed screen allows for identification of reproductive modes of seed formation and inference of the ploidy of contributing gametes. However, the lack of a mathematical formalization to infer male/female genomic contributions, and the prerequisite of a binucleate female contribution to the endosperm limits its applicability. We evaluated this assumption combining a DNA-based progeny survey with a comparison of the cytology of reproductive pathways co-occurring within single individuals representing 14 Potentilleae species from six phylogenetic lineages. A numerical framework valid for sexual and pseudogamous taxa was developed, enabling quantification of female and male genomes contributing to embryo and endosperm independent of gametophyte origins, numbers of sperm involved and ploidy of parents. The inference strongly depended on accurate peak index estimation. The endosperm of Potentilleae species received a binucleate female contribution in five evolutionary lineages whereas endosperm formation remained uncertain in the Tormentillae. A modified flow cytometric seed screen protocol was developed to cope with low endosperm contents. Evolutionary conservation of a binucleate female contribution to the endosperm suggested wide applicability of flow cytometric seed screen - at least in the Potentilleae. However, alternative progeny surveys and precise embryo/endosperm ploidy estimates are required for a comprehensive understanding of the cytology of seed formation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.