ArticlePloS one2013
Cyclic nucleotide gated channels 7 and 8 are essential for male reproductive fertility.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 91 citations in OpenAlex.
- CNGCs in Marchantia paleacea uncouple arbuscular mycorrhizal symbiosis and rhizoid development.The New phytologist · 2026Article
- Unravelling the genetics of heat-responsive fruit traits in pepper [Capsicum annuum L.] via GWAS under subtropical climates.BMC plant biology · 2026Article
- Cryo-EM structures of Arabidopsis CNGC1 and CNGC5 reveal molecular mechanisms underlying gating and calcium selectivity.Nature plants · 2025Article
- From gametes to zygote: Mechanistic advances and emerging possibilities in plant reproduction.Plant physiology · 2024Review
- Long small RNA76113 targets CYCLIC NUCLEOTIDE-GATED ION CHANNEL 5 to repress disease resistance in rice.Plant physiology · 2024Article
- Assembly and annotation of the black spruce genome provide insights on spruce phylogeny and evolution of stress response.G3 (Bethesda, Md.) · 2023Article
- Genome-Wide Exploration and Expression Analysis of the CNGC Gene Family in Eggplant (International journal of molecular sciences · 2023Article
- Genome-Wide Analysis and Expression ofPlants (Basel, Switzerland) · 2023Article
- Multiple cyclic nucleotide-gated channels function as ABA-activated Ca2+ channels required for ABA-induced stomatal closure in Arabidopsis.The Plant cell · 2023Article
- The captivating role of calcium in plant-microbe interaction.Frontiers in plant science · 2023Review
- RALF signaling pathway activates MLO calcium channels to maintain pollen tube integrity.Cell research · 2023Article
- Genome-Wide Analysis of Cyclic Nucleotide-Gated Channel Genes Related to Pollen Development in Rice.Plants (Basel, Switzerland) · 2022Article
- A novel calmodulin-interacting Domain of Unknown Function 506 protein represses root hair elongation in Arabidopsis.Plant, cell & environment · 2022Article
- Hexose transporter SWEET5 confers galactose sensitivity to Arabidopsis pollen germination via a galactokinase.Plant physiology · 2022Article
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- Two Is Company, but Four Is a Party-Challenges of Tetraploidization for Cell Wall Dynamics and Efficient Tip-Growth in Pollen.Plants (Basel, Switzerland) · 2021Review
- Links between Regulatory Systems of ROS and Carbohydrates in Reproductive Development.Plants (Basel, Switzerland) · 2021Review
- The Complex Story of Plant Cyclic Nucleotide-Gated Channels.International journal of molecular sciences · 2021Review
- Plant Cyclic Nucleotide-Gated Channels: New Insights on Their Functions and Regulation.Plant physiology · 2020Article
- Calcium spikes, waves and oscillations in plant development and biotic interactions.Nature plants · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 3 countries.
Funding
Abstract
The Arabidopsis thaliana genome contains 20 CNGCs, which are proposed to encode cyclic nucleotide gated, non-selective, Ca²⁺-permeable ion channels. CNGC7 and CNGC8 are the two most similar with 74% protein sequence identity, and both genes are preferentially expressed in pollen. Two independent loss-of-function T-DNA insertions were identified for both genes and used to generate plant lines in which only one of the two alleles was segregating (e.g., cngc7-1+/-/cngc8-2-/- and cngc7-3-/-/cngc8-1+/-). While normal pollen transmission was observed for single gene mutations, pollen harboring mutations in both cngc7 and 8 were found to be male sterile (transmission efficiency reduced by more than 3000-fold). Pollen grains harboring T-DNA disruptions of both cngc7 and 8 displayed a high frequency of bursting when germinated in vitro. The male sterile defect could be rescued through pollen expression of a CNGC7 or 8 transgene including a CNGC7 with an N-terminal GFP-tag. However, rescue efficiencies were reduced ∼10-fold when the CNGC7 or 8 included an F to W substitution (F589W and F624W, respectively) at the junction between the putative cyclic nucleotide binding-site and the calmodulin binding-site, identifying this junction as important for proper functioning of a plant CNGC. Using confocal microscopy, GFP-CNGC7 was found to preferentially localize to the plasma membrane at the flanks of the growing tip. Together these results indicate that CNGC7 and 8 are at least partially redundant and provide an essential function at the initiation of pollen tube tip growth.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.