ArticleThe Plant cell2013
Arabidopsis CSN5B interacts with VTC1 and modulates ascorbic acid synthesis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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72 citing papers in PubMed, 154 citations in OpenAlex.
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- Overexpression of soybean flavonoid 3'-hydroxylase enhances plant salt tolerance by promoting ascorbic acid biosynthesis.Journal of advanced research · 2026Article
- 2-(3,4-Dichlorophenoxy)triethylamine (DCPTA) Sustains Root Activity Through the Enhancement of Ascorbate-Glutathione in Spring Maize (International journal of molecular sciences · 2025Article
- SmCSN5 is a synergist in the transcription factor SmMYB36-mediated biosynthesis of tanshinones and phenolic acids inHorticulture research · 2025Article
- Viral-Based Gene Editing System for Nutritional Improvement of Fructan Content in Lettuce.International journal of molecular sciences · 2025Article
- The Silencing of theInternational journal of molecular sciences · 2025Article
- The Regulation of ROS and Phytohormones in Balancing Crop Yield and Salt Tolerance.Antioxidants (Basel, Switzerland) · 2025Review
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- The zinc finger protein DHHC09 S-acylates the kinase STRK1 to regulate H2O2 homeostasis and promote salt tolerance in rice.The Plant cell · 2024Article
- Deciphering the Genomic Characterization of the GGP Gene Family and Expression Verification ofAntioxidants (Basel, Switzerland) · 2024Article
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- OsJAB1 Positively Regulates Ascorbate Biosynthesis and Negatively Regulates Salt Tolerance Due to Inhibiting Early-Stage Salt-Induced ROS Accumulation in Rice.Plants (Basel, Switzerland) · 2023Article
- Genome-wide association study unveils ascorbate regulation by PAS/LOV PROTEIN during high light acclimation.Plant physiology · 2023Article
- L-Ascorbic acid metabolism and regulation in fruit crops.Plant physiology · 2023Article
- Blue light promotes ascorbate synthesis by deactivating the PAS/LOV photoreceptor that inhibits GDP-L-galactose phosphorylase.The Plant cell · 2023Article
- Receptor-Like Cytoplasmic Kinase STK Confers Salt Tolerance in Rice.Rice (New York, N.Y.) · 2023Article
- Ascorbic acid-mediated reactive oxygen species homeostasis modulates the switch from tapetal cell division to cell differentiation in Arabidopsis.The Plant cell · 2023Article
- Plant deubiquitinases: from structure and activity to biological functions.Plant cell reports · 2023Review
12 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Light regulates ascorbic acid (AsA) synthesis, which increases in the light, presumably reflecting a need for antioxidants to detoxify reactive molecules produced during photosynthesis. Here, we examine this regulation in Arabidopsis thaliana and find that alterations in the protein levels of the AsA biosynthetic enzyme GDP-Man pyrophosphorylase (VTC1) are associated with changes in AsA contents in light and darkness. To find regulatory factors involved in AsA synthesis, we identified VTC1-interacting proteins by yeast two-hybrid screening of a cDNA library from etiolated seedlings. This screen identified the photomorphogenic factor COP9 signalosome subunit 5B (CSN5B), which interacted with the N terminus of VTC1 in yeast and plants. Gel filtration profiling showed that VTC1-CSN5B also associated with the COP9 signalosome complex, and this interaction promotes ubiquitination-dependent VTC1 degradation through the 26S proteasome pathway. Consistent with this, csn5b mutants showed very high AsA levels in both light and darkness. Also, a double mutant of csn5b with the partial loss-of-function mutant vtc1-1 contained AsA levels between those of vtc1-1 and csn5b, showing that CSN5B modulates AsA synthesis by affecting VTC1. In addition, the csn5b mutant showed higher tolerance to salt, indicating that CSN5B regulation of AsA synthesis affects the response to salt stress. Together, our data reveal a regulatory role of CSN5B in light-dark regulation of AsA synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.