ArticleJournal of cell science2013
Mechanisms of CDC-42 activation during contact-induced cell polarization.
Article in Journal of cell science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
32 citing papers in PubMed, 50 citations in OpenAlex.
- LGL-1 and the RhoGAP protein PAC-1 redundantly polarize thebioRxiv : the preprint server for biology · 2026Article
- The actin assembly regulatorbioRxiv : the preprint server for biology · 2025Article
- Article
- A computational analysis of the role of integrins and Rho-GTPases in the emergence and disruption of apical-basal polarization in renal epithelial cells.PLoS computational biology · 2024Article
- Origin and development of primary animal epithelia.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Apical PAR protein caps orient the mitotic spindle in C. elegans early embryos.Current biology : CB · 2023Article
- Actin and CDC-42 contribute to nuclear migration through constricted spaces in C. elegans.Development (Cambridge, England) · 2023Article
- FLN-2 functions in parallel to LINC complexes and Cdc42/actin pathways during P-cell nuclear migration through constricted spaces inbioRxiv : the preprint server for biology · 2023Article
- Computational study of biomechanical drivers of renal cystogenesis.Biomechanics and modeling in mechanobiology · 2023Article
- The RhoGEF protein Plekhg5 regulates medioapical and junctional actomyosin dynamics of apical constriction duringMolecular biology of the cell · 2023Article
- Apical PAR-3 caps orient the mitotic spindle inbioRxiv : the preprint server for biology · 2023Article
- Aurora A and cortical flows promote polarization and cytokinesis by inducing asymmetric ECT-2 accumulation.eLife · 2022Article
- A caspase-RhoGEF axis contributes to the cell size threshold for apoptotic death in developing Caenorhabditis elegans.PLoS biology · 2022Article
- Article
- Review
- TheGenetics · 2019Review
- Planar Asymmetries in theFrontiers in cell and developmental biology · 2019Article
- The RhoGEF protein Plekhg5 regulates apical constriction of bottle cells during gastrulation.Development (Cambridge, England) · 2018Article
- Article
- PAR-Complex and Crumbs Function During Photoreceptor Morphogenesis and Retinal Degeneration.Frontiers in cellular neuroscience · 2018Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Polarization of early embryos provides a foundation to execute essential patterning and morphogenetic events. In Caenorhabditis elegans, cell contacts polarize early embryos along their radial axis by excluding the cortical polarity protein PAR-6 from sites of cell contact, thereby restricting PAR-6 to contact-free cell surfaces. Radial polarization requires the cortically enriched Rho GTPase CDC-42, which in its active form recruits PAR-6 through direct binding. The Rho GTPase activating protein (RhoGAP) PAC-1, which localizes specifically to cell contacts, triggers radial polarization by inactivating CDC-42 at these sites. The mechanisms responsible for activating CDC-42 at contact-free surfaces are unknown. Here, in an overexpression screen of Rho guanine nucleotide exchange factors (RhoGEFs), which can activate Rho GTPases, we identify CGEF-1 and ECT-2 as RhoGEFs that act through CDC-42 to recruit PAR-6 to the cortex. We show that ECT-2 and CGEF-1 localize to the cell surface and that removing their activity causes a reduction in levels of cortical PAR-6. Through a structure-function analysis, we show that the tandem DH-PH domains of CGEF-1 and ECT-2 are sufficient for GEF activity, but that regions outside of these domains target each protein to the cell surface. Finally, we provide evidence suggesting that the N-terminal region of ECT-2 may direct its in vivo preference for CDC-42 over another known target, the Rho GTPase RHO-1. We propose that radial polarization results from a competition between RhoGEFs, which activate CDC-42 throughout the cortex, and the RhoGAP PAC-1, which inactivates CDC-42 at cell contacts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.