ArticleMethods in molecular biology (Clifton, N.J.)2013
In vitro evolution of enzymes.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Adaptive evolution of oriC through in vitro propagation of a mini-chromosome in RCR.Nucleic acids research · 2025Article
- Unveiling the new chapter in nanobody engineering: advances in traditional construction and AI-driven optimization.Journal of nanobiotechnology · 2025Review
- Automated in vivo enzyme engineering accelerates biocatalyst optimization.Nature communications · 2024Review
- DNA methylases for site-selective inhibition of type IIS restriction enzyme activity.Applied microbiology and biotechnology · 2024Article
- Evolution and synthetic biology.Current opinion in microbiology · 2023Review
- Review
- Lymphocytes in Cellular Therapy: Functional Regulation of CAR T Cells.Frontiers in immunology · 2018Review
- Extensive libraries of gene truncation variants generated by in vitro transposition.Nucleic acids research · 2017Article
- Peptide aptamers: development and applications.Current topics in medicinal chemistry · 2015Review
- Article
- Highly diverse protein library based on the ubiquitous (β/α)₈ enzyme fold yields well-structured proteins through in vitro folding selection.Chembiochem : a European journal of chemical biology · 2013Article
- Universal labeling of 5'-triphosphate RNAs by artificial RNA ligase enzyme with broad substrate specificity.Chemical communications (Cambridge, England) · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
In the past decade, in vitro evolution techniques have been used to improve the performance or alter the activity of a number of different enzymes and have generated enzymes de novo. In this review, we provide an overview of the available in vitro methods, their application, and some general considerations for enzyme engineering in vitro. We discuss the advantages of in vitro over in vivo approaches and focus on ribosome display, mRNA display, DNA display technologies, and in vitro compartmentalization (IVC) methods. This review aims to help researchers determine which approach is best suited for their own experimental needs and to highlight that in vitro methods offer a promising route for enzyme engineering.
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Registered trials
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