ArticleInvestigative ophthalmology & visual science2013
A new mouse model of inducible, chronic retinal ganglion cell dysfunction not associated with cell death.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Using Noninvasive Electrophysiology to Determine Time Windows of Neuroprotection in Optic Neuropathies.International journal of molecular sciences · 2022Article
- Mitochondrial Transfer of the Mutant HumanTranslational vision science & technology · 2020Article
- Target-Derived Neurotrophic Factor Deprivation Puts Retinal Ganglion Cells on Death Row: Cold Hard Evidence and Caveats.International journal of molecular sciences · 2019Review
- Anesthetic Preconditioning as Endogenous Neuroprotection in Glaucoma.International journal of molecular sciences · 2018Article
- Evaluating retinal ganglion cell loss and dysfunction.Experimental eye research · 2016Review
- Integrative properties of retinal ganglion cell electrical responsiveness depend on neurotrophic support and genotype in the mouse.Experimental eye research · 2016Article
- Brain derived neurotrophic factor keeps pattern electroretinogram from dropping after superior colliculus lesion in mice.International journal of ophthalmology · 2016Article
- Electrophysiological assessment of retinal ganglion cell function.Experimental eye research · 2015Review
- Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure.Journal of neuroinflammation · 2015Article
- Frequency spectrum might act as communication code between retina and visual cortex I.International journal of ophthalmology · 2015Article
- Robust mouse pattern electroretinograms derived simultaneously from each eye using a common snout electrode.Investigative ophthalmology & visual science · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo develop a mouse model of inducible, chronic retinal ganglion cell (RGC) dysfunction not associated with cell death.
methodsEighteen C57BL/6J mice were longitudinally tested with pattern electroretinogram (PERG) and spectral-domain optical coherence tomography (OCT) before and after aspiration of the contralateral superior colliculus (SC), which removed terminals of optic tract axons and the superficial layers of the SC. At the 4-month end points, retinas were harvested for Brn3b immunostaining and BDNF immunoblotting.
resultsThe PERG lost approximately 60% of its baseline amplitude (P < 0.01) within the first day after lesion, and remained at a reduced level over 4 months. At the end point, the density of Brn3b-positive RGCs was normal, but their nucleus size was reduced by approximately 24% (P < 001). OCT measurements showed thinning of the inner, but not outer, retina by approximately 9% (P < 0.01) starting 10 to 20 days after lesion. Retinal nerve fiber layer thickness was unchanged. At the end point, retinal homogenates showed a substantial overexpression of BDNF protein level.
conclusionsMechanical SC lesion in adult mice results in a rapid, chronic loss of RGC electrical responsiveness that is followed by cell shrinkage but not cell death. The SC-lesion mouse represents a new, inducible model that allows investigating stages and mechanisms of RGC dysfunction without the confounding effects of cell death that are common in the existing models of optic neuropathies and optic nerve lesions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.