ArticleNature communications2013
Cleavage-based signal amplification of RNA.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 87 citations in OpenAlex.
- Value of Blood-Based microRNAs in the Diagnosis of Acute Myocardial Infarction: A Systematic Review and Meta-Analysis.Frontiers in physiology · 2020Pooled it
- Pooled it
- The 10-23 DNAzyme in Biosensing and Diagnostics: Applications, Challenges, and Future Directions.Angewandte Chemie (International ed. in English) · 2026Review
- Interface-constrained catalytic hairpin assembly permits highly sensitive SERS signaling of miRNA.Mikrochimica acta · 2024Article
- Recent Advances in Design and Application of Nanomaterials-Based Colorimetric Biosensors for Agri-food Safety Analysis.ACS omega · 2023Review
- Positive feedback drives a secondary nonlinear product burst during a biphasic DNA amplification reaction.The Analyst · 2022Article
- Types and Applications of Nicking Enzyme-Combined Isothermal Amplification.International journal of molecular sciences · 2022Review
- Unified-amplifier based primer exchange reaction (UniAmPER) enabled detection of SARS-CoV-2 from clinical samples.Sensors and actuators. B, Chemical · 2022Article
- A Combinational Approach for More Efficient miRNA Biosensing.Current genomics · 2022Review
- Biosensing with DNAzymes.Chemical Society reviews · 2021Review
- Site-specific functionalization with amino, guanidinium, and imidazolyl groups enabling the activation of 10-23 DNAzyme.RSC advances · 2020Article
- Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis.Biochemistry. Biokhimiia · 2020Review
- A mathematical model for a biphasic DNA amplification reaction.Journal of the Royal Society, Interface · 2019Article
- Artificial Signal Feedback Network Mimicking Cellular Adaptivity.Journal of the American Chemical Society · 2019Article
- First characterization of a biphasic, switch-like DNA amplification.The Analyst · 2018Article
- Multiplex quantitative analysis of microRNA expression via exponential isothermal amplification and conformation-sensitive DNA separation.Scientific reports · 2017Article
- Integrating Deoxyribozymes into Colorimetric Sensing Platforms.Sensors (Basel, Switzerland) · 2016Review
- Label-Free Isothermal Amplification Assay for Specific and Highly Sensitive Colorimetric miRNA Detection.ACS omega · 2016Article
- Nucleic acid detection using G-quadruplex amplification methodologies.Methods (San Diego, Calif.) · 2013Review
- Direct visualization of the reaction transformation and signal amplification in a DNA molecular machine with total internal reflection fluorescence microscopy.Frontiers in chemistry · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA detection has become an integral part of current biomedical research. Up to now, the reverse transcription-PCR has been the most practical method to detect mRNA targets. However, RNA detection by reverse transcription-PCR requires sophisticated equipment and it is highly sensitive to contamination with genomic DNA. Here we report a new isothermal reaction to simultaneously amplify and detect RNA, based on cleavage by DNAzyme and signal amplification. Cleavage-based signal amplification of RNA cannot be contaminated by genomic DNA and is suitable for the detection of both mRNA and microRNA targets, with high specificity and sensitivity. Moreover, the detection results can be reported in a colorimetric or real-time fluorometric way for different detection purposes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.