ArticleNature chemistry2013
Volume-conserving trans-cis isomerization pathways in photoactive yellow protein visualized by picosecond X-ray crystallography.
Article in Nature chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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85 citing papers in PubMed.
- Liquid-Microjet Photoelectron Spectroscopy of the Photoactive Yellow Protein Chromophore in Aqueous Solution.The journal of physical chemistry. A · 2026Article
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- A Bright and Dynamic Future in Structural Biology.Advances in experimental medicine and biology · 2026Review
- Advances in Crystallography and Cryo-Electron Microscopy for the Study of Membrane Proteins.Advances in experimental medicine and biology · 2026Review
- Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization.IUCrJ · 2025Article
- Deconvolution of dynamic heterogeneity in protein structure.Structural dynamics (Melville, N.Y.) · 2024Review
- Photoinduced dual bond rotation of a nitrogen-containing system realized by chalcogen substitution.Nature chemistry · 2024Article
- Dynamic three-dimensional structures of a metal-organic framework captured with femtosecond serial crystallography.Nature chemistry · 2024Article
- KINNTREX: a neural network to unveil protein mechanisms from time-resolved X-ray crystallography.IUCrJ · 2024Article
- Watching a signaling protein function: What has been learned over four decades of time-resolved studies of photoactive yellow protein.Structural dynamics (Melville, N.Y.) · 2024Review
- The time revolution in macromolecular crystallography.Structural dynamics (Melville, N.Y.) · 2024Article
- From femtoseconds to minutes: time-resolved macromolecular crystallography at XFELs and synchrotrons.Acta crystallographica. Section D, Structural biology · 2024Article
- Blue and red in the protein world: Photoactive yellow protein and phytochromes as revealed by time-resolved crystallography.Structural dynamics (Melville, N.Y.) · 2024Article
- The TR-icOS setup at the ESRF: time-resolved microsecond UV-Vis absorption spectroscopy on protein crystals.Acta crystallographica. Section D, Structural biology · 2024Article
- Heterogeneity in M. tuberculosis β-lactamase inhibition by Sulbactam.Nature communications · 2023Article
- Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism.Journal of the American Chemical Society · 2023Article
- Mechanistic Elucidation of the Hula-Twist Photoreaction in Hemithioindigo.Journal of the American Chemical Society · 2023Article
- Practical considerations for the analysis of time-resolved x-ray data.Structural dynamics (Melville, N.Y.) · 2023Article
- Projection to extract the perpendicular component (PEPC) method for extracting kinetics from time-resolved data.Structural dynamics (Melville, N.Y.) · 2023Article
- Heterogeneity in the M. tuberculosis β-Lactamase Inhibition by Sulbactam.Research square · 2023Article
25 more citing papers are in PubMed but not listed here.
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Abstract
Trans-to-cis isomerization, the key reaction in photoactive proteins, usually cannot occur through the standard one-bond-flip mechanism. Owing to spatial constraints imposed by a protein environment, isomerization probably proceeds through a volume-conserving mechanism in which highly choreographed atomic motions are expected, the details of which have not yet been observed directly. Here we employ time-resolved X-ray crystallography to visualize structurally the isomerization of the p-coumaric acid chromophore in photoactive yellow protein with a time resolution of 100 ps and a spatial resolution of 1.6 Å. The structure of the earliest intermediate (I(T)) resembles a highly strained transition state in which the torsion angle is located halfway between the trans- and cis-isomers. The reaction trajectory of I(T) bifurcates into two structurally distinct cis intermediates via hula-twist and bicycle-pedal pathways. The bifurcating reaction pathways can be controlled by weakening the hydrogen bond between the chromophore and an adjacent residue through E46Q mutation, which switches off the bicycle-pedal pathway.
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