ArticleMethods in enzymology2013
Protein switch engineering by domain insertion.
Article in Methods in enzymology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- State-of-the-art in engineering small molecule biosensors and their applications in metabolic engineering.SLAS technology · 2024Review
- Living electronics: A catalogue of engineered living electronic components.Microbial biotechnology · 2023Review
- Shotgun scanning glycomutagenesis: A simple and efficient strategy for constructing and characterizing neoglycoproteins.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Engineering proteins for allosteric control by light or ligands.Nature protocols · 2019Article
- Plant Synthetic Biology: Quantifying the "Known Unknowns" and Discovering the "Unknown Unknowns".Plant physiology · 2019Review
- Circular permutation profiling by deep sequencing libraries created using transposon mutagenesis.Nucleic acids research · 2018Article
- Incorporating an allosteric regulatory site in an antibody through backbone design.Protein science : a publication of the Protein Society · 2017Article
- Development of a cancer-marker activated enzymatic switch from the herpes simplex virus thymidine kinase.Protein engineering, design & selection : PEDS · 2017Article
- Engineering of temperature- and light-switchable Cas9 variants.Nucleic acids research · 2016Article
- Characterizing Aciniform Silk Repetitive Domain Backbone Dynamics and Hydrodynamic Modularity.International journal of molecular sciences · 2016Article
- Modular protein switches derived from antibody mimetic proteins.Protein engineering, design & selection : PEDS · 2016Article
- Insertion of a xylanase in xylose binding protein results in a xylose-stimulated xylanase.Biotechnology for biofuels · 2015Article
- A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase.Molecular systems biology · 2014Article
- Advancements in the development of HIF-1α-activated protein switches for use in enzyme prodrug therapy.PloS one · 2014Article
- The designability of protein switches by chemical rescue of structure: mechanisms of inactivation and reactivation.Journal of the American Chemical Society · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The switch-like regulation of protein activity by molecular signals is abundant in native proteins. The ability to engineer proteins with novel regulation has applications in biosensors, selective protein therapeutics, and basic research. One approach to building proteins with novel switch properties is creating combinatorial libraries of gene fusions between genes encoding proteins that have the prerequisite input and output functions of the desired switch. These libraries are then subjected to selections and/or screens to identify those rare gene fusions that encode functional switches. Combinatorial libraries in which an insert gene is inserted randomly into an acceptor gene have been useful for creating switches, particularly when combined with circular permutation of the insert gene. Methods for creating random domain insertion libraries are described. Three methods for creating a diverse set of insertion sites in the acceptor gene are presented and compared: DNase I digestion, S1 nuclease digestion, and multiplex inverse PCR. A PCR-based method for creating a library of circular permutations of the insert gene is also presented.
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