ArticleRNA biology2013
A two-stage mechanism of viral RNA compaction revealed by single molecule fluorescence.
Article in RNA biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Long ssRNA undergoes continuous compaction in the presence of polyvalent cations.Biophysical journal · 2023Article
- Single-particle studies of the effects of RNA-protein interactions on the self-assembly of RNA virus particles.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Compositional complementarity between genomic RNA and coat proteins in positive-sense single-stranded RNA viruses.Nucleic acids research · 2022Article
- In vitro functional analysis of gRNA sites regulating assembly of hepatitis B virus.Communications biology · 2021Article
- The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA.Nature communications · 2021Article
- Article
- RNA-Mediated Virus Assembly: Mechanisms and Consequences for Viral Evolution and Therapy.Annual review of biophysics · 2019Review
- Packaging of Genomic RNA in Positive-Sense Single-Stranded RNA Viruses: A Complex Story.Viruses · 2019Review
- RNA: packaged and protected by VLPs.RSC advances · 2018Article
- Alphavirus Nucleocapsid Packaging and Assembly.Viruses · 2018Review
- Article
- Genomic RNA folding mediates assembly of human parechovirus.Nature communications · 2017Article
- Sizes of Long RNA Molecules Are Determined by the Branching Patterns of Their Secondary Structures.Biophysical journal · 2016Article
- Asymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ.Nature communications · 2016Article
- Direct Evidence for Packaging Signal-Mediated Assembly of Bacteriophage MS2.Journal of molecular biology · 2016Article
- Article
- Evidence that avian reovirus σNS is an RNA chaperone: implications for genome segment assortment.Nucleic acids research · 2015Article
- Revealing the density of encoded functions in a viral RNA.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Solving a Levinthal's paradox for virus assembly identifies a unique antiviral strategy.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Proteome-wide analysis reveals clues of complementary interactions between mRNAs and their cognate proteins as the physicochemical foundation of the genetic code.RNA biology · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Long RNAs often exist as multiple conformers in equilibrium. For the genomes of single-stranded RNA viruses, one of these conformers must include a compacted state allowing the RNA to be confined within the virion. We have used single molecule fluorescence correlation spectroscopy to monitor the conformations of viral genomes and sub-fragments in the absence and presence of coat proteins. Cognate RNA-coat protein interactions in two model viruses cause a rapid collapse in the hydrodynamic radii of their respective RNAs. This is caused by protein binding at multiple sites on the RNA that facilitate additional protein-protein contacts. The collapsed species recruit further coat proteins to complete capsid assembly with great efficiency and fidelity. The specificity in RNA-coat protein interactions seen at single-molecule concentrations reflects the packaging selectivity seen for such viruses in vivo. This contrasts with many in vitro reassembly measurements performed at much higher concentrations. RNA compaction by coat protein or polycation binding are distinct processes, implying that defined RNA-coat protein contacts are required for assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.