Evidence map›Paper›PMID 23421680›Full record

ArticleACS chemical neuroscience2013

trans-Resveratrol protects ischemic PC12 Cells by inhibiting the hypoxia associated transcription factors and increasing the levels of antioxidant defense enzymes.

Megha Agrawal, Vivek Kumar, Abhishek K Singh, Mahendra P Kashyap, Vinay K Khanna, Maqsood A Siddiqui, Aditya B Pant

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS chemical neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 57 citations in OpenAlex.

  1. Article
  2. Article
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  4. Article
  5. The Nrf2 Pathway in Ischemic Stroke: A Review.Molecules (Basel, Switzerland) · 2021
    Review
  6. Article
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  9. Electroacupuncture Ameliorates Cerebral I/R-Induced Inflammation through DOR-BDNF/TrkB Pathway.Evidence-based complementary and alternative medicine : eCAM · 2020
    Article
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  12. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Megha AgrawalCSIR-Indian Institute of Toxicology Research, Lucknow, India.
Vivek Kumar
Abhishek K Singh
Mahendra P Kashyap
Vinay K Khanna
Maqsood A Siddiqui
Aditya B Pant
Indian Institute of Toxicology Research · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An in vitro model of ischemic cerebral stroke [oxygen-glucose deprivation (OGD) for 6 h followed by 24 h reoxygenation (R)] with PC12 cells increases Ca(2+) influx by upregulating native L-type Ca(2+) channels and reactive oxygen species (ROS) generation. This reactive oxygen species generation and increase in intracellular Ca(2+) triggers the expression of hypoxic homeostasis transcription factors such as hypoxia induced factor-1 alpha (HIF-1α), Cav-beta 3 (Cav β3), signal transducer and activator of transcription 3 (STAT3), heat shock protein 27 (hsp-27), and cationic channel transient receptor potential melastatin 7 (TRPM7). OGD insulted PC12 cells were subjected to biologically safe doses (5, 10, and 25 μM) of trans-resveratrol in three different treatment groups: 24 h prior to OGD (pre-treatment); 24 h post OGD (post-treatment); and from 24 h before OGD to end of reoxygenation period (whole-treatment). Here, we demonstrated that OGD-R-induced neuronal injury/death is by reactive oxygen species generation, increase in intracellular calcium levels, and decrease in antioxidant defense enzymes. trans-Resveratrol increases the viability of OGD-R insulted PC12 cells, which was assessed by using MTT, NRU, and LDH release assay. In addition, trans-resveratrol significantly decreases reactive oxygen species generation, intracellular Ca(2+) levels, and hypoxia associated transcription factors and also increases the level of antioxidant defense enzymes. Our data shows that the whole-treatment group of trans-resveratrol is most efficient in decreasing hypoxia induced cell death through its antioxidant properties.

Indexed as

AnimalsAntioxidantsCalciumCalcium ChannelsCell DeathCell HypoxiaHSP27 Heat-Shock ProteinsHypoxiaHypoxia-Inducible Factor 1, alpha SubunitIschemiaL-Lactate DehydrogenasePC12 CellsRatsReactive Oxygen SpeciesResveratrolSTAT3 Transcription FactorAntioxidantsCacnb3 protein, ratCalciumCalcium ChannelsHif1a protein, ratHSP27 Heat-Shock ProteinsHspb1 protein, ratHypoxia-Inducible Factor 1, alpha SubunitL-Lactate DehydrogenaseReactive Oxygen SpeciesResveratrolSod1 protein, ratStat3 protein, ratSTAT3 Transcription FactorStilbenesSuperoxide DismutaseSuperoxide Dismutase-1Trpm7 protein, ratTRPM Cation Channels

Identifiers

PMID23421680
PMCPMC3582290
OpenAlexW1971020412

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.