ArticleThe Journal of physiology2013
Regulation of connexin36 gap junction channels by n-alkanols and arachidonic acid.
Article in The Journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 35 citations in OpenAlex.
- Defining the electrical synapse.Nature reviews. Neuroscience · 2026Review
- Rod/Cone Gap Junctions: Plastic Electrical Synapses at the First Visual Synapse.Annual review of vision science · 2026Review
- Quantifying electrostatic control of docking and binding energetics in functional Cx36 gap junctions.Communications biology · 2026Article
- Lipids intercalate and mediate multi-channel assemblies of connexin-46/50 gap junctions.bioRxiv : the preprint server for biology · 2026Article
- Dose- and Time-Dependent Modulation of Cx43 and Cx45 Expression and Gap Junction Conductance by Resveratrol.Antioxidants (Basel, Switzerland) · 2026Article
- Mefloquine-induced conformational shift in Cx36 N-terminal helix leading to channel closure mediated by lipid bilayer.Nature communications · 2024Article
- The components of an electrical synapse as revealed by expansion microscopy of a single synaptic contact.eLife · 2024Article
- Cryo-EM structures of human Cx36/GJD2 neuronal gap junction channel.Nature communications · 2023Article
- When microscopy and electrophysiology meet connectomics-Steve Massey's contribution to unraveling the structure and function of the rod/cone gap junction.Frontiers in ophthalmology · 2023Review
- Analysis of rod/cone gap junctions from the reconstruction of mouse photoreceptor terminals.eLife · 2022Article
- The Amino Terminal Domain and Modulation of Connexin36 Gap Junction Channels by Intracellular Magnesium Ions.Frontiers in physiology · 2022Article
- Prostaglandin E2 Enhances Gap Junctional Intercellular Communication in Clonal Epithelial Cells.International journal of molecular sciences · 2021Article
- Role of Lipid Metabolism and Signaling in Mammalian Oocyte Maturation, Quality, and Acquisition of Competence.Frontiers in cell and developmental biology · 2021Review
- Beyond plasticity: the dynamic impact of electrical synapses on neural circuits.Nature reviews. Neuroscience · 2019Review
- The SH3-binding domain of Cx43 participates in loop/tail interactions critical for Cx43-hemichannel activity.Cellular and molecular life sciences : CMLS · 2018Article
- Molecular basis for potentiation of Cx36 gap junction channel conductance byBioscience reports · 2018Article
- Connexins in Cardiovascular and Neurovascular Health and Disease: Pharmacological Implications.Pharmacological reviews · 2017Review
- The electrical synapse: Molecular complexities at the gap and beyond.Developmental neurobiology · 2017Review
- Regulation of Connexin-Based Channels by Fatty Acids.Frontiers in physiology · 2017Review
- From Neuron Biophysics to Orientation Selectivity in Electrically Coupled Networks of Neocortical L2/3 Large Basket Cells.Cerebral cortex (New York, N.Y. : 1991) · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
We examined junctional conductance (gj) and its dependence on transjunctional voltage in gap junction (GJ) channels formed of wild-type connexin36 (Cx36) or its fusion form with green fluorescent protein (Cx36-EGFP) transfected in HeLa cells or endogenously expressed in primary culture of pancreatic β-cells. Only a very small fraction (∼0.8%) of Cx36-EGFP channels assembled into junctional plaques of GJs were open under control conditions. We found that short carbon chain n-alkanols (SCCAs) increased gj, while long carbon chain n-alkanols resulted in full uncoupling; cutoff is between heptanol and octanol. The fraction of functional channels and gj increased several fold under an exposure to SCCAs, or during reduction of endogenous levels of arachidonic acid (AA) by exposure to fatty acid-free BSA or cytosolic phospholipase A2 inhibitors. Moreover, uncoupling caused by exogenously applied AA can be rescued by BSA, which binds AA and other polyunsaturated fatty acids (PUFAs), but not by BSA modified with 1,2-cyclohexanedione, which does not bind AA and other PUFAs. We propose that under control conditions, Cx36 GJ channels in HeLa transfectants and β-cells are inhibited by endogenous AA, which stabilizes a closed conformational state of the channel that leads to extremely low fraction of functional channels. In addition, SCCAs increase gj by interfering with endogenous AA-dependent inhibition, increasing open probability and the fraction of functional channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.