ArticleJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry2013
Reprogramming EF-hands for design of catalytically amplified lanthanide sensors.
Article in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Article
- Redox-Responsive Protein Design: Design of a Small Protein Motif Dependent on Glutathionylation.Biochemistry · 2018Article
- A Designed Enzyme Promotes Selective Post-translational Acylation.Chembiochem : a European journal of chemical biology · 2018Article
- Design of an allosterically regulated retroaldolase.Protein science : a publication of the Protein Society · 2015Article
- Catalytic efficiency of designed catalytic proteins.Current opinion in structural biology · 2014Review
- Design of catalytically amplified sensors for small molecules.Biomolecules · 2014Review
- A single mutation in a regulatory protein produces evolvable allosterically regulated catalyst of nonnatural reaction.Angewandte Chemie (International ed. in English) · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We recently reported that a computationally designed catalyst nicknamed AlleyCat facilitates C-H proton abstraction in Kemp elimination at neutral pH in a selective and calcium-dependent fashion by a factor of approximately 100,000 (Korendovych et al. in Proc. Natl. Acad. Sci. USA 108:6823, 2011). Kemp elimination produced a colored product that can be easily read out, thus making AlleyCat a catalytically amplified metal sensor for calcium. Here we report that metal-binding EF-hand motifs in AlleyCat could be redesigned to incorporate trivalent metal ions without significant loss of catalytic activity. Mutation of a single neutral residue at position 9 of each of the EF-hands to glutamate results in almost a two orders of magnitude improvement of selectivity for trivalent metal ions over calcium. Development of this new lanthanide-dependent switchable Kemp eliminase, named CuSeCat EE, provides the foundation for further selectivity improvement and broadening the scope of the repertoire of metals for sensing. A concerted effort in the design of switchable enzymes has many environmental, sensing, and metal ion tracking applications.
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