ArticleThe journal of physical chemistry letters2012
Signal Discrimination Between Fluorescent Proteins in Live Cells by Long-wavelength Optical Modulation.
Article in The journal of physical chemistry letters, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Optically Modulated and Optically Activated Delayed Fluorescent Proteins through Dark State Engineering.The journal of physical chemistry. B · 2021Article
- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Facile autofluorescence suppression enabling tracking of single viruses in live cells.The Journal of biological chemistry · 2019Article
- Improved Fluorescent Protein Contrast and Discrimination by Optically Controlling Dark State Lifetimes.The journal of physical chemistry letters · 2017Article
- The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.Trends in biochemical sciences · 2017Review
- Dark State-Modulated Fluorescence Correlation Spectroscopy for Quantitative Signal Recovery.The journal of physical chemistry letters · 2016Article
- Optically Modulated Photoswitchable Fluorescent Proteins Yield Improved Biological Imaging Sensitivity.Journal of the American Chemical Society · 2015Article
- Expanding discriminative dimensions for analysis and imaging.Chemical science · 2015Article
- Molecular approaches to chromatography using single molecule spectroscopy.Analytical chemistry · 2015Review
- Optically modulated fluorescence bioimaging: visualizing obscured fluorophores in high background.Accounts of chemical research · 2014Review
- Optically modulatable blue fluorescent proteins.Journal of the American Chemical Society · 2013Article
- Modulated fluorophore signal recovery buried within tissue mimicking phantoms.The journal of physical chemistry. A · 2013Article
- DNA-Templated Molecular Silver Fluorophores.The journal of physical chemistry letters · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescent proteins (FPs) have revolutionized molecular and cellular biology; yet, discrimination over cellular autofluorescence, spectral deconvolution, or detection at low concentrations remain challenging problems in many biological applications. By optically depopulating a photoinduced dark state with orange secondary laser co-excitation, the higher-energy green AcGFP fluorescence is dynamically increased. Modulating this secondary laser then modulates the higher-energy, collected fluorescence; enabling its selective detection by removing heterogeneous background from other FPs. Order-of-magnitude reduction in obscuring fluorophore background emission has been achieved in both fixed and live cells. This longwavelength modulation expands the dimensionality to discriminate FP emitters based on dark state lifetimes and enables signal of interest to be recovered by removing heterogeneous background emitter signals. Thus, AcGFP is not only useful for extracting weak signals from systems plagued by high background, but it is a springboard for further FP optimization and utilization for improving sensitivity and selectivity in biological fluorescence imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.