ArticleJournal of cell science2013
Tetraspanin18 is a FoxD3-responsive antagonist of cranial neural crest epithelial-to-mesenchymal transition that maintains cadherin-6B protein.
Article in Journal of cell science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Genetic heterogeneity and homogeneity among orofacial cleft subtypes: genome-wide association studies in the cleft collective.Human molecular genetics · 2025Pooled it
- Neural crest gene regulatory networks as drivers of development, diversification and disease.Nature reviews. Molecular cell biology · 2026Review
- From neural crest migration to the onset of gangliogenesis.Current topics in developmental biology · 2025Review
- TSPAN18 facilitates bone metastasis of prostate cancer by protecting STIM1 from TRIM32-mediated ubiquitination.Journal of experimental & clinical cancer research : CR · 2023Article
- Thyroid hormone membrane receptor binding and transcriptional regulation in the sea urchinFrontiers in endocrinology · 2023Article
- Time to go: neural crest cell epithelial-to-mesenchymal transition.Development (Cambridge, England) · 2022Review
- Chick cranial neural crest cells release extracellular vesicles that are critical for their migration.Journal of cell science · 2022Article
- Tspan18 is a novel regulator of thrombo-inflammation.Medical microbiology and immunology · 2020Review
- Disrupted ER membrane protein complex-mediated topogenesis drives congenital neural crest defects.The Journal of clinical investigation · 2020Article
- Rehabilitative Impact of Exercise Training on Human Skeletal Muscle Transcriptional Programs in Parkinson's Disease.Frontiers in physiology · 2020Article
- Tspan18 is a novel regulator of the CaHaematologica · 2019Article
- Cellular organization and boundary formation in craniofacial development.Genesis (New York, N.Y. : 2000) · 2019Review
- Cadherin-6B proteolytic N-terminal fragments promote chick cranial neural crest cell delamination by regulating extracellular matrix degradation.Developmental biology · 2018Article
- Leukocyte receptor tyrosine kinase interacts with secreted midkine to promote survival of migrating neural crest cells.Development (Cambridge, England) · 2018Article
- Specifying neural crest cells: From chromatin to morphogens and factors in between.Wiley interdisciplinary reviews. Developmental biology · 2018Review
- A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices.Mechanisms of development · 2018Article
- Should I stay or should I go? Cadherin function and regulation in the neural crest.Genesis (New York, N.Y. : 2000) · 2017Review
- The Wnt receptor Frizzled-4 modulates ADAM13 metalloprotease activity.Journal of cell science · 2015Article
- Establishing neural crest identity: a gene regulatory recipe.Development (Cambridge, England) · 2015Review
- Expression and Subcellular Distribution of GFP-Tagged Human Tetraspanin Proteins in Saccharomyces cerevisiae.PloS one · 2015Article
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2 authors.
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Abstract
During epithelial-to-mesenchymal transition (EMT), tightly associated, polarized epithelial cells become individual mesenchymal cells capable of migrating. Here, we investigate the role of the transmembrane protein tetraspanin18 (Tspan18) in chick cranial neural crest EMT. Tspan18 mRNA is expressed in premigratory cranial neural crest cells, but is absent from actively migrating neural crest cells. Tspan18 knockdown leads to a concomitant loss of cadherin-6B (Cad6B) protein, whereas Cad6B protein persists when Tspan18 expression is extended. The temporal profile of Cad6B mRNA downregulation is unaffected in these embryos, which indicates that Tspan18 maintains Cad6B protein levels and reveals that Cad6B is regulated by post-translational mechanisms. Although downregulation of Tspan18 is necessary, it is not sufficient for neural crest migration: the timing of neural crest emigration, basal lamina breakdown and Cad7 upregulation proceed normally in Tspan18-deficient cells. This emphasizes the need for coordinated transcriptional and post-translational regulation of Cad6B during EMT and illustrates that Tspan18-antagonized remodeling of cell-cell adhesions is only one step in preparation for cranial neural crest migration. Unlike Cad6B, which is transcriptionally repressed by Snail2, Tspan18 expression is downstream of the winged-helix transcription factor FoxD3, providing a new transcriptional input into cranial neural crest EMT. Together, our data reveal post-translational regulation of Cad6B protein levels by Tspan18 that must be relieved by a FoxD3-dependent mechanism in order for cranial neural crest cells to migrate. These results offer new insight into the molecular mechanisms of cranial neural crest EMT and expand our understanding of tetraspanin function relevant to metastasis.
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