ArticlePlanta2013
Global transcriptome analysis and identification of a CONSTANS-like gene family in the orchid Erycina pusilla.
Article in Planta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Evolution of major flowering pathway integrators in Orchidaceae.Plant reproduction · 2024Article
- Combined Transcriptomic and Metabolomic Approach Revealed a Relationship between Light Control, Photoprotective Pigments, and Lipid Biosynthesis in Olives.International journal of molecular sciences · 2023Article
- Transcriptome mining of hormonal and floral integrators in the leafless flowers of three cymbidium orchids.Frontiers in plant science · 2022Article
- Genetic insights into the regulatory pathways for continuous flowering in a unique orchid Arundina graminifolia.BMC plant biology · 2021Article
- Organ-Specific Gene Expression Reveals the Role of theFrontiers in plant science · 2021Article
- Morphological and Molecular Characterization of Orchid Fruit Development.Frontiers in plant science · 2019Article
- Photoperiod response-related gene SiCOL1 contributes to flowering in sesame.BMC plant biology · 2018Article
- Comparative transcriptomics provides insight into the molecular basis of species diversification of section Trigonopedia (Cypripedium) on the Qinghai-Tibetan Plateau.Scientific reports · 2018Article
- Post genomics era for orchid research.Botanical studies · 2017Review
- GBS-based single dosage markers for linkage and QTL mapping allow gene mining for yield-related traits in sugarcane.BMC genomics · 2017Article
- Transcriptome-wide analysis of the MADS-box gene family in the orchid Erycina pusilla.Plant biotechnology journal · 2016Article
- Article
- Global transcriptome analysis and identification of the flowering regulatory genes expressed in leaves of Lagerstroemia indica.DNA and cell biology · 2014Article
- BeMADS1 is a key to delivery MADSs into nucleus in reproductive tissues-De novo characterization of Bambusa edulis transcriptome and study of MADS genes in bamboo floral development.BMC plant biology · 2014Article
- Shedding some light over the floral metabolism by arum lily (Zantedeschia aethiopica) spathe de novo transcriptome assembly.PloS one · 2014Article
- De novo transcriptome assembly from inflorescence of Orchis italica: analysis of coding and non-coding transcripts.PloS one · 2014Article
- The analysis of the inflorescence miRNome of the orchid Orchis italica reveals a DEF-like MADS-box gene as a new miRNA target.PloS one · 2014Article
- Catalog of Erycina pusilla miRNA and categorization of reproductive phase-related miRNAs and their target gene families.Plant molecular biology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The high chromosome numbers, polyploid genomes, and long juvenile phases of most ornamental orchid species render functional genomics difficult and limit the discovery of genes influencing horticultural traits. The orchid Erycina pusilla has a low chromosome number (2n = 12) and flowers in vitro within 1 year, making it a standout candidate for use as a model orchid. However, transcriptomic and genomic information from E. pusilla remains limited. In this study, next-generation sequencing (NGS) technology was used to identify 90,668 unigenes by de novo assembly. These unigenes were annotated functionally and analyzed with regard to their gene ontology (GO), clusters of orthologous groups (COG), and KEGG pathways. To validate the discovery methods, a homolog of CONSTANS (CO), one of the key genes in the flowering pathway, was further analyzed. The Arabidopsis CO-Like (COL) amino acid sequences were used to screen for homologs in the E. pusilla transcriptome database. Specific primers to the homologous unigenes were then used to isolate BAC clones, which were sequenced to identify 12 E. pusilla CO-like (EpCOL) full-length genes. Based on sequence homology, domain structure, and phylogenetic analysis, these EpCOL genes were divided into four groups. Four EpCOLs fused with GFP were localized in the nucleus. Some EpCOL genes were regulated by light. These results demonstrate that nascent E. pusilla resources (transcriptome and BAC library) can be used to investigate the E. pusilla photoperiod-dependent flowering genes. In future, this strategy can be applied to other biological processes, marketable traits, and molecular breeding in this model orchid.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.