ArticleApplied and environmental microbiology2013
Legionella pneumophila transcriptional response following exposure to CuO nanoparticles.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Effects of Copper onPathogens (Basel, Switzerland) · 2024Article
- Exposure of Legionella pneumophila to low-shear modeled microgravity: impact on stress response, membrane lipid composition, pathogenicity to macrophages and interrelated genes expression.Archives of microbiology · 2024Article
- Quantification of silver nanoparticle interactions with yeast Saccharomyces cerevisiae studied using single-cell ICP-MS.Analytical and bioanalytical chemistry · 2022Article
- Molecular Mechanisms of Nanomaterial-Bacterial Interactions Revealed by Omics-The Role of Nanomaterial Effect Level.Frontiers in bioengineering and biotechnology · 2021Review
- Mitigation of Expression of Virulence Genes inPathogens (Basel, Switzerland) · 2020Article
- Electrically heatable carbon nanotube point-of-use filters for effective separation and in-situ inactivation ofChemical engineering journal (Lausanne, Switzerland : 1996) · 2019Article
- The Combined Effect of Cold and Copper Stresses on the Proliferation and Transcriptional Response ofFrontiers in microbiology · 2019Article
- Effect of temperature and colonization of Legionella pneumophila and Vermamoeba vermiformis on bacterial community composition of copper drinking water biofilms.Microbial biotechnology · 2017Article
- Transcriptomic changes of Legionella pneumophila in water.BMC genomics · 2015Article
- Environmental (Saprozoic) Pathogens of Engineered Water Systems: Understanding Their Ecology for Risk Assessment and Management.Pathogens (Basel, Switzerland) · 2015Review
- Exposure to synthetic gray water inhibits amoeba encystation and alters expression of Legionella pneumophila virulence genes.Applied and environmental microbiology · 2015Article
- Analysis of the transcriptome of Legionella pneumophila hfq mutant reveals a new mobile genetic element.Microbiology (Reading, England) · 2013Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
Copper ions are an effective antimicrobial agent used to control Legionnaires' disease and Pontiac fever arising from institutional drinking water systems. Here, we present data on an alternative bactericidal agent, copper oxide nanoparticles (CuO-NPs), and its efficacy on Legionella pneumophila. In broth cultures, the CuO-NPs caused growth inhibition, which appeared to be concentration and exposure time dependent. The transcriptomic response of L. pneumophila to CuO-NP exposure was investigated by using a whole-genome microarray. The expression of genes involved in metabolism, transcription, translation, DNA replication and repair, and unknown/hypothetical proteins was significantly affected by exposure to CuO-NPs. In addition, expression of 21 virulence genes was also affected by exposure to CuO-NP and further evaluated by quantitative reverse transcription-PCR (qRT-PCR). Some virulence gene responses occurred immediately and transiently after addition of CuO-NPs to the cells and faded rapidly (icmV, icmW, lepA), while expression of other genes increased within 6 h (ceg29, legLC8, legP, lem19, lem24, lpg1689, and rtxA), 12 h (cegC1, dotA, enhC, htpX, icmE, pvcA, and sidF), and 24 h (legP, lem19, and ceg19), but for most of the genes tested, expression was reduced after 24 h of exposure. Genes like ceg29 and rtxA appeared to be the most responsive to CuO-NP exposures and along with other genes identified in this study may prove useful to monitor and manage the impact of drinking water disinfection on L. pneumophila.
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Registered trials
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