ArticleApplied and environmental microbiology2013
CYP63A2, a catalytically versatile fungal P450 monooxygenase capable of oxidizing higher-molecular-weight polycyclic aromatic hydrocarbons, alkylphenols, and alkanes.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 96 citations in OpenAlex.
- Structure-Function Analysis of the Self-Sufficient CYP102 Family Provides New Insights into Their Biochemistry.International journal of molecular sciences · 2025Article
- Three strategy rules of filamentous fungi in hydrocarbon remediation: an overview.Biodegradation · 2024Review
- Pezizomycetes Genomes Reveal Diverse P450 Complements Characteristic of Saprotrophic and Ectomycorrhizal Lifestyles.Journal of fungi (Basel, Switzerland) · 2023Article
- Fungal bioproducts for petroleum hydrocarbons and toxic metals remediation: recent advances and emerging technologies.Bioprocess and biosystems engineering · 2023Review
- Filamentous fungi for sustainable remediation of pharmaceutical compounds, heavy metal and oil hydrocarbons.Frontiers in bioengineering and biotechnology · 2023Review
- Leveraging Multiple Data Streams for Prioritization of Mixtures for Hazard Characterization.Toxics · 2022Article
- Marginal lands and fungi - linking the type of soil contamination with fungal community composition.Environmental microbiology · 2022Article
- Preparative Production of Functionalized (Biomolecules · 2022Article
- Late-Stage Functionalisation of Polycyclic (Biomolecules · 2021Article
- Evolution and enrichment of CYP5035 in Polyporales: functionality of an understudied P450 family.Applied microbiology and biotechnology · 2021Article
- Ligninolytic enzyme involved in removal of high molecular weight polycyclic aromatic hydrocarbons by Fusarium strain ZH-H2.Environmental science and pollution research international · 2020Article
- An overview of the factors playing a role in cytochrome P450 monooxygenase and ferredoxin interactions.Biophysical reviews · 2020Review
- Simulation of Microbial Response to Accidental Diesel Spills in Basins Containing Brackish Sea Water and Sediment.Frontiers in microbiology · 2020Article
- Distinct Regioselectivity of Fungal P450 Enzymes for Steroidal Hydroxylation.Applied and environmental microbiology · 2019Article
- The CYPome of the model xenobiotic-biotransforming fungus Cunninghamella elegans.Scientific reports · 2019Article
- Comparative Analyses of Cytochrome P450s and Those Associated with Secondary Metabolism inInternational journal of molecular sciences · 2018Article
- Biochemical Characterization of CYP505D6, a Self-Sufficient Cytochrome P450 from the White-Rot Fungus Phanerochaete chrysosporium.Applied and environmental microbiology · 2018Article
- Distribution of Archaeal Communities along the Coast of the Gulf of Finland and Their Response to Oil Contamination.Frontiers in microbiology · 2018Article
- Diverse Metabolic Capacities of Fungi for Bioremediation.Indian journal of microbiology · 2016Review
- Versatile biocatalysis of fungal cytochrome P450 monooxygenases.Microbial cell factories · 2016Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Cytochrome P450 monooxygenases (P450s) are known to oxidize hydrocarbons, albeit with limited substrate specificity across classes of these compounds. Here we report a P450 monooxygenase (CYP63A2) from the model ligninolytic white rot fungus Phanerochaete chrysosporium that was found to possess a broad oxidizing capability toward structurally diverse hydrocarbons belonging to mutagenic/carcinogenic fused-ring higher-molecular-weight polycyclic aromatic hydrocarbons (HMW-PAHs), endocrine-disrupting long-chain alkylphenols (APs), and crude oil aliphatic hydrocarbon n-alkanes. A homology-based three-dimensional (3D) model revealed the presence of an extraordinarily large active-site cavity in CYP63A2 compared to the mammalian PAH-oxidizing (CYP3A4, CYP1A2, and CYP1B1) and bacterial aliphatic-hydrocarbon-oxidizing (CYP101D and CYP102A1) P450s. This structural feature in conjunction with ligand docking simulations suggested potential versatility of the enzyme. Experimental characterization using recombinantly expressed CYP63A2 revealed its ability to oxidize HMW-PAHs of various ring sizes, including 4 rings (pyrene and fluoranthene), 5 rings [benzo(a)pyrene], and 6 rings [benzo(ghi)perylene], with the highest enzymatic activity being toward the 5-ring PAH followed by the 4-ring and 6-ring PAHs, in that order. Recombinant CYP63A2 activity yielded monohydroxylated PAH metabolites. The enzyme was found to also act as an alkane ω-hydroxylase that oxidized n-alkanes with various chain lengths (C9 to C12 and C15 to C19), as well as alkyl side chains (C3 to C9) in alkylphenols (APs). CYP63A2 showed preferential oxidation of long-chain APs and alkanes. To our knowledge, this is the first P450 identified from any of the biological kingdoms that possesses such broad substrate specificity toward structurally diverse xenobiotics (PAHs, APs, and alkanes), making it a potent enzyme biocatalyst candidate to handle mixed pollution (e.g., crude oil spills).
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