ArticleIntegrative biology : quantitative biosciences from nano to macro2013
Manipulating the lateral diffusion of surface-anchored EGF demonstrates that receptor clustering modulates phosphorylation levels.
Article in Integrative biology : quantitative biosciences from nano to macro, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Clustering of Membrane Receptors: Insights from DNA Origami-Based Approaches.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- Surface-Patterned DNA Origami Rulers Reveal Nanoscale Distance Dependency of the Epidermal Growth Factor Receptor Activation.Nano letters · 2024Article
- Manipulation of PD-L1 Endosomal Trafficking Promotes Anticancer Immunity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Critical roles for EGFR and EGFR-HER2 clusters in EGF binding of SW620 human carcinoma cells.Journal of the Royal Society, Interface · 2022Article
- Mechanisms of Action of EGFR Tyrosine Kinase Receptor Incorporated in Extracellular Vesicles.Cells · 2020Review
- Membrane receptor activation mechanisms and transmembrane peptide tools to elucidate them.The Journal of biological chemistry · 2020Review
- Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells.Science (New York, N.Y.) · 2020Article
- Leader cell PLCγ1 activation during keratinocyte collective migration is induced by EGFR localization and clustering.Bioengineering & translational medicine · 2019Article
- Characterizing Large-Scale Receptor Clustering on the Single Cell Level: A Comparative Plasmon Coupling and Fluorescence Superresolution Microscopy Study.The journal of physical chemistry. B · 2019Article
- Topography design in model membranes: Where biology meets physics.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- The extracellular matrix as a key regulator of intracellular signalling networks.British journal of pharmacology · 2019Review
- Proteins on Supported Lipid Bilayers Diffusing around Proteins Fixed on Acrylate Anchors.Analytical chemistry · 2018Article
- Ligand Density and Nanoparticle Clustering Cooperate in the Multivalent Amplification of Epidermal Growth Factor Receptor Activation.ACS nano · 2018Article
- Mechanisms of epidermal growth factor receptor signaling as characterized by patterned ligand activation and mutational analysis.Biochimica et biophysica acta. Biomembranes · 2017Review
- MYO6 Regulates Spatial Organization of Signaling Endosomes Driving AKT Activation and Actin Dynamics.Cell reports · 2017Article
- Critical importance of appropriate fixation conditions for faithful imaging of receptor microclusters.Biology open · 2016Article
- Supramolecular Nanofibers Enhance Growth Factor Signaling by Increasing Lipid Raft Mobility.Nano letters · 2016Article
- Plasma Membrane Organization of Epidermal Growth Factor Receptor in Resting and Ligand-Bound States.Biophysical journal · 2015Article
- The significance of membrane fluidity of feeder cell-derived substrates for maintenance of iPS cell stemness.Scientific reports · 2015Article
- GRB2 Nucleates T Cell Receptor-Mediated LAT Clusters That Control PLC-γ1 Activation and Cytokine Production.Frontiers in immunology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Upon activation, the epidermal growth factor (EGF) receptor becomes phosphorylated and triggers a vast signaling network that has profound effects on cell growth. The EGF receptor is observed to assemble into clusters after ligand binding and tyrosine kinase autophosphorylation, but the role of these assemblies in the receptor signaling pathway remains unclear. To address this question, we measured the phosphorylation of EGFR when the EGF ligand was anchored onto laterally mobile and immobile surfaces. We found that cells generated clusters of ligand-receptor complex on mobile EGF surfaces, and displayed a lower ratio of phosphorylated EGFR to EGF when compared to immobilized EGF that is unable to cluster. This result was verified by tuning the lateral assembly of ligand-receptor complexes on the surface of living cells using patterned supported lipid bilayers. Nanoscale metal lines fabricated into the supported membrane constrained lipid diffusion and EGF receptor assembly into micron and sub-micron scale corrals. Single cell analysis indicated that clustering impacts EGF receptor activation, and larger clusters (>1 μm(2)) of ligand-receptor complex generated lower EGF receptor phosphorylation per ligand than smaller assemblies (<1 μm(2)) in HCC1143 cells that were engaged to ligand-functionalized surfaces. We investigated the mechanism of EGFR clustering by treating cells with compounds that disrupt the cytoskeleton (Latrunculin B), clathrin-mediated endocytosis (Pitstop2), and inhibit EGFR activation (Gefitinib). These results help elucidate the nature of large-scale EGFR clustering, thus underscoring the general significance of receptor spatial organization in tuning biochemical function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.