ArticleCell reports2013
Chimeras reveal a single lipid-interface residue that controls MscL channel kinetics as well as mechanosensitivity.
Article in Cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Structural basis of the gating mechanism of the large-conductance mechanosensitive channel from Escherichia coli.Nature communications · 2026Article
- Asymmetric Nature of MscL Opening Revealed by Molecular Dynamics Simulations.Journal of chemical information and modeling · 2025Article
- Response of heterologously expressed pressure sensor-actuator-modulator macromolecule to external mechanical stress.Heliyon · 2024Article
- Mechanosensitive channel MscL gating transitions coupling with constriction point shift.Protein science : a publication of the Protein Society · 2024Article
- Article
- Charge-voltage curves of Shaker potassium channel are not hysteretic at steady state.The Journal of general physiology · 2023Article
- Asymmetric effects of amphipathic molecules on mechanosensitive channels.Scientific reports · 2022Article
- Advances in space microbiology.iScience · 2021Review
- Mechanical Activation of MscL Revealed by a Locally Distributed Tension Molecular Dynamics Approach.Biophysical journal · 2021Article
- Exploring the diversity of mechanosensitive channels in bacterial genomes.European biophysics journal : EBJ · 2021Article
- Role of adhesion forces in mechanosensitive channel gating in Staphylococcus aureus adhering to surfaces.NPJ biofilms and microbiomes · 2020Article
- Life with Bacterial Mechanosensitive Channels, from Discovery to Physiology to Pharmacological Target.Microbiology and molecular biology reviews : MMBR · 2020Review
- A new antibiotic with potent activity targets MscL.The Journal of antibiotics · 2015Article
- Scanning MscL Channels with Targeted Post-Translational Modifications for Functional Alterations.PloS one · 2015Article
- Electrostatics at the membrane define MscL channel mechanosensitivity and kinetics.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Dynamics of protein-protein interactions at the MscL periplasmic-lipid interface.Biophysical journal · 2014Article
- Article
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Authors and funding
3 authors.
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Abstract
MscL, the highly conserved bacterial mechanosensitive channel of large conductance, serves as an osmotic "emergency release valve," is among the best-studied mechanosensors, and is a paradigm of how a channel senses and responds to membrane tension. Although all homologs tested thus far encode channel activity, many show functional differences. We tested Escherichia coli and Staphylococcus aureus chimeras and found that the periplasmic region of the protein, particularly E. coli I49 and the equivalent S. aureus F47 at the periplasmic lipid-aqueous interface of the first transmembrane domain, drastically influences both the open dwell time and the threshold of channel opening. One mutant shows a severe hysteresis, confirming the importance of this residue in determining the energy barriers for channel gating. We propose that this site acts similarly to a spring for a clasp knife, adjusting the resistance for obtaining and stabilizing an open or closed channel structure.
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