Evidence map›Paper›PMID 23414562›Full record

ArticleNano letters2013

Quantitative multicolor subdiffraction imaging of bacterial protein ultrastructures in three dimensions.

Andreas Gahlmann, Jerod L Ptacin, Ginni Grover, Sean Quirin, Lexy von Diezmann, Marissa K Lee, Mikael P Backlund, Lucy Shapiro, Rafael Piestun, W E Moerner

Abstract read
In one paragraph

Article in Nano letters, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed.

  1. Review
  2. Article
  3. Organization of Myosin H in the Apical Complex ofbioRxiv : the preprint server for biology · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Breakdown of Langmuir Adsorption Isotherm in Small Closed Systems.Langmuir : the ACS journal of surfaces and colloids · 2024
    Article
  14. Article
  15. Single-molecule imaging in the primary cilium.Methods in cell biology · 2023
    Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. A localized adaptor protein performs distinct functions at theProceedings of the National Academy of Sciences of the United States of America · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Andreas GahlmannDepartment of Chemistry, Stanford University, Stanford, California 94305, United States.
Jerod L Ptacin
Ginni Grover
Sean Quirin
Lexy von Diezmann
Marissa K Lee
Mikael P Backlund
Lucy Shapiro
Rafael Piestun
W E Moerner

Funding

REGULATION OF DIFFERENTIATION IN CAULOBACTERR01GM032506 · NIGMS · YESHIVA UNIVERSITY · PI SHAPIRO, LUCILLE · 1985 to 2014
$7.1M
Subcellular architecture of regulatory protein complexes at the bacterial poleR01GM086196 · NIGMS · STANFORD UNIVERSITY · PI MOERNER, WILLIAM E, SHAPIRO, LUCILLE · 2008 to 2015
$4.7M
DEVELOPMENT CONTROL OF DNA REPLICATION IN CAULOBACTERR01GM051426 · NIGMS · STANFORD UNIVERSITY · PI SHAPIRO, LUCILLE · 1994 to 2013
$3.8M
NIGMS NIH HHS R01 GM032506NIGMS NIH HHS R01GM032506NIGMS NIH HHS R01 GM051426NIGMS NIH HHS R01 GM086196NIGMS NIH HHS R01GM086196
6 · The paper itself

Abstract

We demonstrate quantitative multicolor three-dimensional (3D) subdiffraction imaging of the structural arrangement of fluorescent protein fusions in living Caulobacter crescentus bacteria. Given single-molecule localization precisions of 20-40 nm, a flexible locally weighted image registration algorithm is critical to accurately combine the super-resolution data with <10 nm error. Surface-relief dielectric phase masks implement a double-helix response at two wavelengths to distinguish two different fluorescent labels and to quantitatively and precisely localize them relative to each other in 3D.

Indexed as

Bacterial ProteinsCaulobacter crescentusColorBacterial Proteins

Identifiers

PMID23414562
PMCPMC3599789

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.