ArticleFood and environmental virology2013
Prevalence and genetic diversity of klassevirus in wastewater in Japan.
Article in Food and environmental virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Molecular Detection of Human Salivirus in Italy Through Monitoring of Urban Sewages.Food and environmental virology · 2020Article
- Occurrence of Salivirus in Sewage and River Water Samples in Karaj, Iran.Food and environmental virology · 2019Article
- Quantification and genetic analysis of salivirus/klassevirus in wastewater in Arizona, USA.Food and environmental virology · 2014Article
- Occurrence of Human Cosavirus in Wastewater and River Water in Japan.Food and environmental virology · 2014Article
- Development of a reverse transcription-quantitative PCR assay for detection of salivirus/klassevirus.Applied and environmental microbiology · 2013Article
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Authors and funding
2 authors.
Funding
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Abstract
Klassevirus is a novel virus belonging to the family Picornaviridae. This study examined the prevalence and genetic diversity of klassevirus in wastewater. Raw sewage (100 ml) and secondary-treated sewage (2 l) were collected monthly for 14 months between January 2011 and February 2012 from a wastewater treatment plant in Japan. Klassevirus in the sample was concentrated by the electronegative membrane-vortex method, followed by qualitative detection by means of three types of reverse transcription (RT)-nested polymerase chain reactions (PCRs). Klassevirus was detected in seven of the 14 raw sewage (50 %) and four of the 14 secondary-treated sewage (29 %) samples by the RT-nested PCRs targeting the 2C and/or 3D regions. In contrast, none of the samples tested positive for the virus by the RT-nested PCR targeting the VP0/VP3 region. Based on direct nucleotide sequence analysis of the klassevirus-positive nested PCR fragments, the tested samples showed high nucleotide sequence similarities of 94.7-100.0 % and 93.2-100.0 % in the 2C and 3D regions, respectively, indicating the presence of a single klassevirus strain. To our knowledge, this is the first study evaluating seasonal prevalence and genetic diversity of klassevirus in environmental waters.
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