ArticleNucleus (Austin, Tex.)
Nucleolar stress in Drosophila melanogaster: RNAi-mediated depletion of Nopp140.
Article in Nucleus (Austin, Tex.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- Review
- Nucleolar stress: From development to cancer.Seminars in cell & developmental biology · 2023Review
- Article
- Nucleolar stress inBiology open · 2020Article
- Organelle aging: Lessons from model organisms.Journal of genetics and genomics = Yi chuan xue bao · 2019Review
- Emerging Role of the Nucleolar Stress Response in Autophagy.Frontiers in cellular neuroscience · 2019Review
- Tip60 complex promotes expression of a differentiation factor to regulate germline differentiation in female Drosophila.Molecular biology of the cell · 2018Article
- Comparison of Nuclear Matrix and Mitotic Chromosome Scaffold Proteins inMolecular & cellular proteomics : MCP · 2018Article
- Juvenile hormone and its receptor methoprene-tolerant promote ribosomal biogenesis and vitellogenesis in theThe Journal of biological chemistry · 2017Article
- Loss of Drosophila nucleostemin 2 (NS2) blocks nucleolar release of the 60S subunit leading to ribosome stress.Chromosoma · 2017Article
- Modeling congenital disease and inborn errors of development in Drosophila melanogaster.Disease models & mechanisms · 2016Review
- Emerging roles of nucleolar and ribosomal proteins in cancer, development, and aging.Cellular and molecular life sciences : CMLS · 2015Review
- Deletion of Drosophila Nopp140 induces subcellular ribosomopathies.Chromosoma · 2015Article
- A remarkable career in science-Joseph G. Gall.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Article
- Nucleolar stress with and without p53.Nucleus (Austin, Tex.)Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleolar stress results when ribosome biogenesis is disrupted. An excellent example is the human Treacher Collins syndrome in which the loss of the nucleolar chaperone, Treacle, leads to p53-dependent apoptosis in embryonic neural crest cells and ultimately to craniofacial birth defects. Here, we show that depletion of the related nucleolar phosphoprotein, Nopp140, in Drosophila melanogaster led to nucleolar stress and eventual lethality when multiple tissues were depleted of Nopp140. We used TEM, immuno-blot analysis and metabolic protein labeling to show the loss of ribosomes. Targeted loss of Nopp140 in larval wing discs caused Caspase-dependent apoptosis which eventually led to defects in the adult wings. These defects were not rescued by a p53 gene deletion, as the craniofacial defects were in the murine model of TCS, thus suggesting that apoptosis caused by nucleolar stress in Drosophila is induced by a p53-independent mechanism. Loss of Nopp140 in larval polyploid midgut cells induced premature autophagy as marked by the accumulation of mCherry-ATG8a into autophagic vesicles. We also found elevated phenoloxidase A3 levels in whole larval lysates and within the hemolymph of Nopp140-depleted larvae vs. hemolymph from parental genotype larvae. Phenoloxidase A3 enrichment was coincident with the appearance of melanotic tumors in the Nopp140-depleted larvae. The occurrence of apoptosis, autophagy and phenoloxidase A3 release to the hemolymph upon nucleolar stress correlated well with the demonstrated activation of Jun N-terminal kinase (JNK) in Nopp140-depleted larvae. We propose that JNK is a central stress response effector that is activated by nucleolar stress in Drosophila larvae.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.