ArticleVirology journal2013
Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses.
Article in Virology journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
85 citing papers in PubMed, 1 synthesis or guideline pooled it, 144 citations in OpenAlex.
- Pooled it
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- Diagnosis and Surveillance of West Nile Virus Infection in Horses: Current Methods, Challenges, and Future Directions.Veterinary sciences · 2026Review
- Entomo-virological investigation during the epizootic outbreak of sylvatic yellow fever in Rio Grande do Sul, Brazil, between 2021 and 2022Memorias do Instituto Oswaldo Cruz · 2026Article
- Integrated Surveillance of Viral Pathogens in Wild Boars: Serological and Molecular Insight in Southern Italy.Animals : an open access journal from MDPI · 2026Article
- Characterization of arbovirus infections in patients within Haiti by screening discarded malaria rapid diagnostic test cassettes.PLoS neglected tropical diseases · 2026Article
- Limited IgM seroreactivity to arboviruses in free-ranging black-tufted marmosets (Frontiers in veterinary science · 2026Article
- Sentinel surveillance of acute febrile illness in a Brazilian Amazon region: laboratory findings and epidemiological implications.Revista do Instituto de Medicina Tropical de Sao Paulo · 2026Article
- Novel quantitative and specific RT-qPCR assay for UK subtypes of European strain of Tick-borne encephalitis virus.Virus research · 2025Article
- [Overview of PCR-based Diagnostic Assays for Emerging Infectious Disease Pathogens].Jugan geon-gang gwa jilbyeong · 2025Review
- Yellow Fever Virus in Aedes albopictus Mosquitoes from Urban Green Area, São Paulo State, Brazil.Emerging infectious diseases · 2025Article
- Uncovering Hidden Transmission: Active Surveillance Reveals Cryptic Circulation of Yellow Fever Virus in Urban Marmosets in Belo Horizonte, Brazil, 2024.Pathogens (Basel, Switzerland) · 2025Article
- Metagenomic detection of central nervous system infections missedby conventional testing.JCI insight · 2025Article
- Colorimetric RT-LAMP for SARS-CoV-2 detection from nasopharyngeal swabs or crude saliva: a multicountry diagnostic accuracy study in Africa.The Lancet. Global health · 2025Article
- Transfusion-transmitted infections: risks and mitigation strategies for Oropouche virus and other emerging arboviruses in Latin America and the Caribbean.Lancet regional health. Americas · 2025Review
- Diagnostic challenges of arboviral infections and dengue virus serotype distribution in febrile patients in East Java, Indonesia.IJID regions · 2025Article
- Dengue: epidemiology, diagnosis methods, treatment options, and prevention strategies.Archives of virology · 2025Review
- Excellent capability for molecular detection ofJournal of clinical microbiology · 2025Article
- Neurotropic Tick-Borne Flavivirus in Alpine Chamois (Viruses · 2025Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe genus Flavivirus includes several pathogenic agents that cause severe illness in humans. Re-emergence of West Nile virus in Europe and continuous spread of certain flaviviruses such as dengue, yellow fever and Japanese encephalitis viruses represent a global danger to public health. Therefore, a rapid and accurate molecular method is required for diagnostics and epidemiological surveillance of flaviviruses.
methodsA Pan-Flavi quantitative RT-PCR assay using a Locked-Nucleic Acid probe targeting the flavivirus NS5 gene was developed and optimized to detect a wide range of flaviviruses simultaneously. The specificity and sensitivity of the Pan-Flavi assay were tested using RNA of different flaviviruses and non-flaviviruses. Furthermore, the assay was compared directly to flavivirus species-specific assays for the ability to detect flaviviruses sensitively.
resultsTwo degenerate primers and one Locked-Nucleic Acids probe were designed to amplify most of the flaviviruses. To increase the specificity and fluorescence signal of the Pan-Flavi assay for detection of yellow fever virus and dengue virus 4, additional primers and probes were included. Viral RNA of thirty different flaviviruses was detected, verifying the broad range specificity. The testing of this assay was successful, using standard plasmid and RNA dilutions of yellow fever virus vaccine strain, dengue virus 1 and tick-borne encephalitis virus, with a sensitivity limit of 10-100 genome copies/reaction. Also comparatively good results were achieved for detecting different flaviviruses by the Pan-Flavi assay when compared to the flavivirus species-specific assays.
conclusionThe assay is rapid, broad-range flavivirus-specific and highly sensitive making it a valuable tool for rapid detection of flaviviruses in livestock samples, epidemiological studies or as useful complement to single flavivirus-specific assays for clinical diagnosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.