ArticleBMC infectious diseases2013
Multiplex qPCR for reliable detection and differentiation of Burkholderia mallei and Burkholderia pseudomallei.
Article in BMC infectious diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 30 citations in OpenAlex.
- Laboratory diagnosis of melioidosis.PLoS neglected tropical diseases · 2025Review
- Evaluation of Quadruple Real-Time PCR Method to Detect Enterococci Carrying Vancomycin-Resistant GenesFrontiers in medicine · 2020Article
- Novel Tetraplex Quantitative PCR Assays for Simultaneous Detection and Identification ofFrontiers in plant science · 2019Article
- Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae.Biomolecular detection and quantification · 2016Article
- Recent Advances in Burkholderia mallei and B. pseudomallei Research.Current tropical medicine reports · 2015Article
- The Identification and Differentiation between Burkholderia mallei and Burkholderia pseudomallei Using One Gene Pyrosequencing.International scholarly research notices · 2014Article
- Development and evaluation of a panel of filovirus sequence capture probes for pathogen detection by next-generation sequencing.PloS one · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBurkholderia mallei and B. pseudomallei are two closely related species of highly virulent bacteria that can be difficult to detect. Pathogenic Burkholderia are endemic in many regions worldwide and cases of infection, sometimes brought by travelers from unsuspected regions, also occur elsewhere. Rapid, sensitive methods for identification of B. mallei and B. pseudomallei are urgently needed in the interests of patient treatment and epidemiological surveillance.
methodsSignature sequences for sensitive, specific detection of pathogenic Burkholderia based on published genomes were identified and a qPCR assay was designed and validated.
resultsA single-reaction quadruplex qPCR assay for the detection of pathogenic Burkholderia, which includes a marker for internal control of DNA extraction and amplification, was developed. The assay permits differentiation of B. mallei and B. pseudomallei strains, and probit analysis showed a very low detection limit. Use of a multicopy signature sequence permits detection of less than 1 genome equivalent per reaction.
conclusionsThe new assay permits rapid detection of pathogenic Burkholderia and combines enhanced sensitivity, species differentiation, and inclusion of an internal control for both DNA extraction and PCR amplification.
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