ArticlePloS one2013
Generation of an artificial double promoter for protein expression in Bacillus subtilis through a promoter trap system.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Engineering Bacillus subtilis for high-value bioproduction: recent advances and applications.Microbial cell factories · 2025Review
- AdvancingMicroorganisms · 2024Review
- Bacillus subtilis: current and future modification strategies as a protein secreting factory.World journal of microbiology & biotechnology · 2024Review
- Design of a sorbitol-activated nitrogen metabolism-dependent regulatory system for redirection of carbon metabolism flow in Bacillus licheniformis.Nucleic acids research · 2023Article
- A Programmable CRISPR/Cas9 Toolkit Improves Lycopene Production in Bacillus subtilis.Applied and environmental microbiology · 2023Article
- Improved biosynthesis of heme in Bacillus subtilis through metabolic engineering assisted fed-batch fermentation.Microbial cell factories · 2023Article
- An outlook to sophisticated technologies and novel developments for metabolic regulation in theFrontiers in bioengineering and biotechnology · 2023Review
- Engineering ofInternational journal of molecular sciences · 2022Article
- Article
- Analysis of Xylose Operon fromInternational journal of molecular sciences · 2022Article
- Enhancement of edeine production in Brevibacillus brevis X23 via in situ promoter engineering.Microbial biotechnology · 2022Article
- Overexpression ofFrontiers in microbiology · 2022Article
- Internal Promoters and Their Effects on the Transcription of Operon Genes for Epothilone Production inFrontiers in bioengineering and biotechnology · 2021Article
- Construction of a High-Expression System in Bacillus through Transcriptomic Profiling and Promoter Engineering.Microorganisms · 2020Article
- High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters.BMC microbiology · 2019Article
- Yeast Expression Systems: Overview and Recent Advances.Molecular biotechnology · 2019Review
- Design of a high-efficiency synthetic system for l-asparaginase production inEngineering in life sciences · 2019Article
- Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond.World journal of microbiology & biotechnology · 2018Review
- Characterization and high-efficiency secreted expression in Bacillus subtilis of a thermo-alkaline β-mannanase from an alkaliphilic Bacillus clausii strain S10.Microbial cell factories · 2018Article
- Enhanced Expression of Pullulanase inFrontiers in microbiology · 2018Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus subtilis is an attractive host for production of recombinant proteins. Promoters and expression plasmid backbones have direct impacts on the efficiency of gene expression. To screen and isolate strong promoters, a promoter trap vector pShuttleF was developed in this study. Using the vector, approximately 1000 colonies containing likely promoters from Bacillus licheniformis genomic DNA were obtained. Amongst them, pShuttle-09 exhibited the highest β-Gal activities in both Escherichia coli and B. subtilis. The activity of pShuttle-09 in B. subtilis was eight times of that of the P43 promoter, a commonly used strong promoter for B. subtilis. A sequence analysis showed that pShuttle-09 contained P(luxS) and truncated luxS in-frame fused with the reporter gene as well as another fragment upstream of P(luxS) containing a putative promoter. This putative promoter was a hybrid promoter and its β-Gal activity was higher than P(luxS). Reconstructing the hybrid promoter from pShuttle-09 to P(lapS) further improved the β-Gal production by 60%. The usefulness of our promoter trap system is likely due to random shuffling and recombination of DNA fragments and adoption of a rapid and high-throughput screening. Thus, our data provide additional evidence to support the concept of using a promoter trap system to create new promoters.
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