ArticlePloS one2013
Analysis of the enzymatic properties of a broad family of alanine aminotransferases.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
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- Alanine metabolism mediates energy allocation of the brown planthopper to adapt to resistant rice.Journal of advanced research · 2025Article
- Field performance and nitrous oxide emissions of transgenic nitrogen use efficient rice lines cultivated in tropical paddy fields.Transgenic research · 2024Article
- Importance of Michaelis Constants for Cancer Cell Redox Balance and Lactate Secretion-Revisiting the Warburg Effect.Cancers · 2024Review
- Origin and Function of Amino Acids in Nectar and Nectaries ofPlants (Basel, Switzerland) · 2023Article
- Genome-Wide Identification of Candidate Genes Associated with Heat Stress in Mulberry (Current issues in molecular biology · 2023Article
- Review
- CRISPR screens in physiologic medium reveal conditionally essential genes in human cells.Cell metabolism · 2021Article
- A different detection method reveals a new role of alanine aminotransferase as an indicator of liver fibrosis.The Korean journal of internal medicine · 2020Article
- Expression of a cucumber alanine aminotransferase2 gene improves nitrogen use efficiency in transgenic rice.Journal, genetic engineering & biotechnology · 2019Article
- ThePlant direct · 2019Article
- Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds.Frontiers in plant science · 2016Article
- One-step biosynthesis of α-ketoisocaproate from L-leucine by an Escherichia coli whole-cell biocatalyst expressing an L-amino acid deaminase from Proteus vulgaris.Scientific reports · 2015Article
- Alanine aminotransferase variants conferring diverse NUE phenotypes in Arabidopsis thaliana.PloS one · 2015Article
- Assessing the metabolic impact of nitrogen availability using a compartmentalized maize leaf genome-scale model.Plant physiology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alanine aminotransferase (AlaAT) has been studied in a variety of organisms due to the involvement of this enzyme in mammalian processes such as non-alcoholic hepatocellular damage, and in plant processes such as C4 photosynthesis, post-hypoxic stress response and nitrogen use efficiency. To date, very few studies have made direct comparisons of AlaAT enzymes and fewer still have made direct comparisons of this enzyme across a broad spectrum of organisms. In this study we present a direct kinetic comparison of glutamate:pyruvate aminotransferase (GPAT) activity for seven AlaATs and two glutamate:glyoxylate aminotransferases (GGAT), measuring the K(M) values for the enzymes analyzed. We also demonstrate that recombinant expression of AlaAT enzymes in Eschericia coli results in differences in bacterial growth inhibition, supporting previous reports of AlaAT possessing bactericidal properties, attributed to lipopolysaccharide endotoxin recognition and binding. A probable lipopolysaccharide binding region within the AlaAT enzymes, homologous to a region of a lipopolysaccharide binding protein (LBP) in humans, was also identified in this study. The AlaAT enzyme differences identified here indicate that AlaAT homologues have differentiated significantly and the roles these homologues play in vivo may also have diverged significantly. Specifically, the differing kinetics of AlaAT enzymes and how this may alter the nitrogen use efficiency in plants is discussed.
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