ArticlePLoS genetics2013
Drosophila Yemanuclein and HIRA cooperate for de novo assembly of H3.3-containing nucleosomes in the male pronucleus.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 53 citations in OpenAlex.
- Epigenetic inheritance and gene expression regulation in early Drosophila embryos.EMBO reports · 2024Review
- Multiple Roles of dXNP and dADD1-International journal of molecular sciences · 2023Review
- Article
- Reduced histone gene copy number disruptsbioRxiv : the preprint server for biology · 2023Article
- Histone Chaperones as Cardinal Players in Development.Frontiers in cell and developmental biology · 2022Review
- CHD1 controls H3.3 incorporation in adult brain chromatin to maintain metabolic homeostasis and normal lifespan.Cell reports · 2021Article
- HIRA contributes to zygote formation in mice and is implicated in human 1PN zygote phenotype.Reproduction (Cambridge, England) · 2021Article
- Histone variant H3.3 residue S31 is essential for Xenopus gastrulation regardless of the deposition pathway.Nature communications · 2020Article
- Article
- A versatile mouse model of epitope-tagged histone H3.3 to study epigenome dynamics.The Journal of biological chemistry · 2019Article
- Lysine 27 of replication-independent histone H3.3 is required for Polycomb target gene silencing but not for gene activation.PLoS genetics · 2019Article
- ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization.Epigenetics & chromatin · 2018Article
- Single Nucleotide Polymorphisms in the HIRA Gene Affect Litter Size in Small Tail Han Sheep.Animals : an open access journal from MDPI · 2018Article
- The Histone Variant H3.3 in Transcriptional Regulation and Human Disease.Journal of molecular biology · 2017Review
- A Molecular Prospective for HIRA Complex Assembly and H3.3-Specific Histone Chaperone Function.Journal of molecular biology · 2017Review
- The Drosophila DAXX-Like Protein (DLP) Cooperates with ASF1 for H3.3 Deposition and Heterochromatin Formation.Molecular and cellular biology · 2017Article
- Epigenetic modifications and reprogramming in paternal pronucleus: sperm, preimplantation embryo, and beyond.Cellular and molecular life sciences : CMLS · 2017Review
- The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana.Genome biology · 2017Article
- Thioredoxin-dependent disulfide bond reduction is required for protamine eviction from sperm chromatin.Genes & development · 2016Article
- Unlocking sperm chromatin at fertilization requires a dedicated egg thioredoxin in Drosophila.Nature communications · 2016Article
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Authors and funding
10 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The differentiation of post-meiotic spermatids in animals is characterized by a unique reorganization of their nuclear architecture and chromatin composition. In many species, the formation of sperm nuclei involves the massive replacement of nucleosomes with protamines, followed by a phase of extreme nuclear compaction. At fertilization, the reconstitution of a nucleosome-based paternal chromatin after the removal of protamines requires the deposition of maternally provided histones before the first round of DNA replication. This process exclusively uses the histone H3 variant H3.3 and constitutes a unique case of genome-wide replication-independent (RI) de novo chromatin assembly. We had previously shown that the histone H3.3 chaperone HIRA plays a central role for paternal chromatin assembly in Drosophila. Although several conserved HIRA-interacting proteins have been identified from yeast to human, their conservation in Drosophila, as well as their actual implication in this highly peculiar RI nucleosome assembly process, is an open question. Here, we show that Yemanuclein (YEM), the Drosophila member of the Hpc2/Ubinuclein family, is essential for histone deposition in the male pronucleus. yem loss of function alleles affect male pronucleus formation in a way remarkably similar to Hira mutants and abolish RI paternal chromatin assembly. In addition, we demonstrate that HIRA and YEM proteins interact and are mutually dependent for their targeting to the decondensing male pronucleus. Finally, we show that the alternative ATRX/XNP-dependent H3.3 deposition pathway is not involved in paternal chromatin assembly, thus underlining the specific implication of the HIRA/YEM complex for this essential step of zygote formation.
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