ArticlePLoS genetics2013
The Mub1/Ubr2 ubiquitin ligase complex regulates the conserved Dsn1 kinetochore protein.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Native yeast kinetochore structures identify an essential inner kinetochore interaction.Nature communications · 2026Article
- Kinetochore-microtubule attachments are strengthened by Cnn1 stabilization of Stu2.bioRxiv : the preprint server for biology · 2026Article
- Deposition of CENP-ACse4 is enhanced by mutations in the AAA+ ATPase domain of ATAD2Yta7.Genetics · 2026Article
- Native yeast kinetochore structures identify an essential inner kinetochore interaction.bioRxiv : the preprint server for biology · 2026Article
- Keeping it centered: decoding the activities that regulate yeast histone H3 variant Cse4 and confine it to centromeres.Frontiers in cell and developmental biology · 2026Review
- Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.G3 (Bethesda, Md.) · 2022Article
- Kre28-Spc105 interaction is essential for Spc105 loading at the kinetochore.Open biology · 2022Article
- Article
- Cell-cycle phospho-regulation of the kinetochore.Current genetics · 2021Review
- The Proteomic Landscape of Centromeric Chromatin Reveals an Essential Role for the Ctf19Current biology : CB · 2021Article
- Aurora B-dependent Ndc80 degradation regulates kinetochore composition in meiosis.Genes & development · 2020Article
- Glucose Signaling Is Connected to Chromosome Segregation Through Protein Kinase A Phosphorylation of the Dam1 Kinetochore Subunit inGenetics · 2019Article
- Regulation of kinetochore configuration during mitosis.Current genetics · 2018Review
- An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast.eLife · 2018Article
- Purification of kinetochores from the budding yeast Saccharomyces cerevisiae.Methods in cell biology · 2018Article
- Synergistic Control of Kinetochore Protein Levels by Psh1 and Ubr2.PLoS genetics · 2016Article
- Synthetic physical interactions map kinetochore regulators and regions sensitive to constitutive Cdc14 localization.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Intra-tumor genetic heterogeneity and alternative driver genetic alterations in breast cancers with heterogeneous HER2 gene amplification.Genome biology · 2015Article
- Protein purification technique that allows detection of sumoylation and ubiquitination of budding yeast kinetochore proteins Ndc10 and Ndc80.Journal of visualized experiments : JoVE · 2015Article
- A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability.PLoS genetics · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The kinetochore is the macromolecular complex that assembles onto centromeric DNA and orchestrates the segregation of duplicated chromosomes. More than 60 components make up the budding yeast kinetochore, including inner kinetochore proteins that bind to centromeric chromatin and outer proteins that directly interact with microtubules. However, little is known about how these components assemble into a functional kinetochore and whether there are quality control mechanisms that monitor kinetochore integrity. We previously developed a method to isolate kinetochore particles via purification of the conserved Dsn1 kinetochore protein. We find that the Mub1/Ubr2 ubiquitin ligase complex associates with kinetochore particles through the CENP-C(Mif2) protein. Although Mub1/Ubr2 are not stable kinetochore components in vivo, they regulate the levels of the conserved outer kinetochore protein Dsn1 via ubiquitylation. Strikingly, a deletion of Mub1/Ubr2 restores the levels and viability of a mutant Dsn1 protein, reminiscent of quality control systems that target aberrant proteins for degradation. Consistent with this, Mub1/Ubr2 help to maintain viability when kinetochores are defective. Together, our data identify a previously unknown regulatory mechanism for the conserved Dsn1 kinetochore protein. We propose that Mub1/Ubr2 are part of a quality control system that monitors kinetochore integrity, thus ensuring genomic stability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.