ArticleJournal of clinical microbiology2013
Region of difference 4 in alpine Mycobacterium caprae isolates indicates three variants.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- M. caprae in northern Italy: a comprehensive analysis through whole-genome sequencing on the genetic variability in bovine herds.Veterinary research · 2025Article
- Bovine tuberculosis in Central Ethiopian slaughterhouses and the identification of causative mycobacteria by multiplex real-time PCR.BMC microbiology · 2024Article
- The Potential Risk of International Spread ofJournal of veterinary research · 2022Article
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- Video Endoscopy-Guided Intrabronchial Spray Inoculation ofFrontiers in veterinary science · 2022Article
- Evaluation of PCRAfrican health sciences · 2021Article
- A new nomenclature for the livestock-associatedOpen research Europe · 2021Article
- Population Structure of Mycobacterium bovis in Germany: a Long-Term Study Using Whole-Genome Sequencing Combined with Conventional Molecular Typing Methods.Journal of clinical microbiology · 2020Article
- Review
- An African origin forEvolution, medicine, and public health · 2020Article
- Revised Interpretation of the Hain Lifescience GenoType MTBC To DifferentiateAntimicrobial agents and chemotherapy · 2019Article
- Mycobacterium caprae Infection in Captive Borneo Elephant, Japan.Emerging infectious diseases · 2018Article
- Article
- Multilaboratory Evaluation of a Novel Lateral Flow Immunochromatographic Assay for Confirming Isolation of Mycobacterium bovis from Veterinary Diagnostic Specimens.Journal of clinical microbiology · 2017Article
- Investigation of intra-herd spread of Mycobacterium caprae in cattle by generation and use of a whole-genome sequence.Veterinary research communications · 2017Article
- Matrixlysis, an improved sample preparation method for recovery of Mycobacteria from animal tissue material.PloS one · 2017Article
- Two alternative DNA extraction methods to improve the detection of Mycobacterium-tuberculosis-complex members in cattle and red deer tissue samples.BMC microbiology · 2016Article
- Article
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9 authors.
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Abstract
The lack of complete genome sequence information for Mycobacterium caprae complicates a robust differentiation of M. caprae and Mycobacterium bovis. In this study, the presence or absence of M. caprae-specific single nucleotide polymorphisms in lepA and gyrB genes was assessed. The region of difference 4 (RD4) was analyzed for the identification and characterization of M. caprae. Molecular characteristics were evaluated in 12 recent M. caprae isolates from livestock and wildlife collected over a 3-year period in Bavaria, Germany. Conventional PCR strategies, sequence analysis of PCR fragments, and data from a next-generation sequencing approach together with variable-number tandem-repeat genotyping were utilized. Single nucleotide polymorphisms in the lepA and gyrB genes indicating the presence of M. caprae were detected in all the isolates. At least three different RD4 variants were found for Alpine M. caprae isolates. The results demonstrate that the RD4 region is rather heterogeneous in M. caprae genomes. As assumed by others, the presence of RD4 is critical for PCR-based differentiation of M. caprae from M. bovis, but in addition, the observed variability of RD4 allows the identification of M. caprae genotypes and may be indicative of a geographical-type appearance.
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