ArticleMolecular & cellular proteomics : MCP2013
The metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 95 citations in OpenAlex.
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- Comparative transcriptome analysis of genes and metabolic pathways involved in sporulation in Ganoderma lingzhi.G3 (Bethesda, Md.) · 2022Article
- The RNA Chaperone Protein Hfq Regulates the Characteristic Sporulation and Insecticidal Activity ofFrontiers in microbiology · 2022Article
- Repressed Central Carbon Metabolism and Its Effect on Related Metabolic Pathways in Cefoperazone/Sulbactam-ResistantFrontiers in microbiology · 2022Article
- The decrotonylase FoSir5 facilitates mitochondrial metabolic state switching in conidial germination ofeLife · 2021Article
- Endospore Appendages: a novel pilus superfamily from the endospores of pathogenic Bacilli.The EMBO journal · 2021Article
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- Which Is Stronger? A Continuing Battle Between Cry Toxins and Insects.Frontiers in microbiology · 2021Review
- The attachment process and physiological properties of Escherichia coli O157:H7 on quartz.BMC microbiology · 2020Article
- Genomics and Proteomics Analyses Revealed Novel Candidate Pesticidal Proteins in a Lepidopteran-ToxicToxins · 2020Article
- Enhancing the insecticidal activity of new Bacillus thuringiensis X023 by copper ions.Microbial cell factories · 2020Article
- Molecular Evidence thatInternational journal of molecular sciences · 2020Article
- Proteome profile changes during poly-hydroxybutyrate intracellular mobilization in gram positive Bacillus cereus tsu1.BMC microbiology · 2020Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus thuringiensis is a well-known entomopathogenic bacterium used worldwide as an environmentally compatible biopesticide. During sporulation, B. thuringiensis accumulates a large number of parasporal crystals consisting of insecticidal crystal proteins (ICPs) that can account for nearly 20-30% of the cell's dry weight. However, the metabolic regulation mechanisms of ICP synthesis remain to be elucidated. In this study, the combined efforts in transcriptomics and proteomics mainly uncovered the following 6 metabolic regulation mechanisms: (1) proteases and the amino acid metabolism (particularly, the branched-chain amino acids) became more active during sporulation; (2) stored poly-β-hydroxybutyrate and acetoin, together with some low-quality substances provided considerable carbon and energy sources for sporulation and parasporal crystal formation; (3) the pentose phosphate shunt demonstrated an interesting regulation mechanism involving gluconate when CT-43 cells were grown in GYS medium; (4) the tricarboxylic acid cycle was significantly modified during sporulation; (5) an obvious increase in the quantitative levels of enzymes and cytochromes involved in energy production via the electron transport system was observed; (6) most F0F1-ATPase subunits were remarkably up-regulated during sporulation. This study, for the first time, systematically reveals the metabolic regulation mechanisms involved in the supply of amino acids, carbon substances, and energy for B. thuringiensis spore and parasporal crystal formation at both the transcriptional and translational levels.
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