ArticlePhotosynthesis research2013
Wavelength-dependent photodamage to Chlorella investigated with a new type of multi-color PAM chlorophyll fluorometer.
Article in Photosynthesis research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- The Fitting of the OJ Phase of Chlorophyll Fluorescence Induction Based on an Analytical Solution and Its Application in Urban Heat Island Research.Plants (Basel, Switzerland) · 2024Article
- Addressing the long-standing limitations of double exponential and non-rectangular hyperbolic models in quantifying light-response of electron transport rates in different photosynthetic organisms under various conditions.Frontiers in plant science · 2024Article
- Exposed anthocyanic leaves of Prunus cerasifera are special shade leaves with high resistance to blue light but low resistance to red light against photoinhibition of photosynthesis.Annals of botany · 2023Article
- On the concepts and correct use of radiometric quantities for assessing the light environment and their application to plant research.Biophysical reviews · 2023Review
- Chlorophyll fluorometry in evaluating photosynthetic performance: key limitations, possibilities, perspectives and alternatives.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Review
- Prying into the green black-box.Photosynthesis research · 2022Review
- Hysteresis light curves: a protocol for characterizing the time dependence of the light response of photosynthesis.Photosynthesis research · 2022Article
- Genetic architecture of photosynthesis energy partitioning as revealed by a genome-wide association approach.Photosynthesis research · 2021Article
- Effects of MPK3 and MPK6 kinases on the chloroplast architecture and function induced by cold acclimation in Arabidopsis.Photosynthesis research · 2021Article
- A theoretical framework of the hybrid mechanism of photosystem II photodamage.Photosynthesis research · 2021Article
- Article
- Heterologous synthesis of chlorophyllBiotechnology for biofuels · 2019Article
- Wavelength shift strategy to enhance lipid productivity ofBiotechnology for biofuels · 2018Article
- Article
- Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy.Scientific reports · 2017Article
- Photoinactivation of Photosystem II in wild-type and chlorophyll b-less barley leaves: which mechanism dominates depends on experimental circumstances.Photosynthesis research · 2015Article
- Photodamage to the oxygen evolving complex of photosystem II by visible light.Scientific reports · 2015Article
- Apparent PS II absorption cross-section and estimation of mean PAR in optically thin and dense suspensions of Chlorella.Photosynthesis research · 2015Article
- Interactions of photosynthesis with genome size and function.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana.Photosynthesis research · 2013Article
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Abstract
A new type of multi-color PAM chlorophyll fluorometer (Schreiber et al. 2012) was applied for measurements of photodamage to photosystem II (PS II) in optically thin suspensions of Chlorella (200 μg Chl l(-1)) in the presence of 1 mM lincomycin. An action spectrum of the relative decrease of F(v)/F(m) in the 440-625 nm range was measured, which not only showed the expected high activity in the blue, but at a lower level also substantial activity above 540 nm. With the same dilute suspension, a PS II absorption spectrum was derived via measurements of the O-I(1) rise kinetics induced by differently colored strong light at defined incident quantum flux densities. After normalization of the two spectra at 625 nm, the relative extent of photodamage at 440-480 nm proved substantially higher than absorption by PS II, whereas the two spectra were close to identical in the 540-625 nm range. Hence, overall photodamage to PS II appears to consist of two components, one of which is due to light absorbed by PS II pigments, whereas the other one is likely to involve direct light absorption by Mn in the oxygen-evolving complex (Hakala et al. Biochim Biophys Acta 1706:68-80, 2005). Based on this rationale, an action spectrum of the Mn mechanism of photodamage was deconvoluted from the overall action spectrum, declining steeply above 480 nm. An almost identical Mn-spectrum was derived by another approach with the PAR of the various colors being adjusted to give identical rates of PS II turnover, PAR (II). The tentative, basic assumption of negligibly small contribution of the Mn mechanism to photodamage above 540 nm was supported by supplementary measurements using an external 665 nm lamp. 665 nm not only gave about two times PS II turnover as compared to 625 nm, but also about two times photodamage.
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