ArticleBiology open2012
Highly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis.
Article in Biology open, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 70 citations in OpenAlex.
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- Dual function model revised by thyroid hormone receptor alpha knockout frogs.General and comparative endocrinology · 2018Review
- Generation of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transfer.Developmental biology · 2017Article
- Expanding the genetic toolkit in Xenopus: Approaches and opportunities for human disease modeling.Developmental biology · 2017Review
- Leapfrogging: primordial germ cell transplantation permits recovery of CRISPR/Cas9-induced mutations in essential genes.Development (Cambridge, England) · 2016Article
- The cellular and molecular mechanisms of tissue repair and regeneration as revealed by studies inRegeneration (Oxford, England) · 2016Article
- Functional joint regeneration is achieved using reintegration mechanism in Xenopus laevis.Regeneration (Oxford, England) · 2016Article
- Functional Cloning Using a Xenopus Oocyte Expression System.Journal of visualized experiments : JoVE · 2016Article
- Xenopus pax6 mutants affect eye development and other organ systems, and have phenotypic similarities to human aniridia patients.Developmental biology · 2015Article
- Manipulation and in vitro maturation of Xenopus laevis oocytes, followed by intracytoplasmic sperm injection, to study embryonic development.Journal of visualized experiments : JoVE · 2015Article
- Development of a new approach for targeted gene editing in primordial germ cells using TALENs in Xenopus.Biology open · 2015Article
- Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis.Biology open · 2015Article
- Comparison of T7E1 and surveyor mismatch cleavage assays to detect mutations triggered by engineered nucleases.G3 (Bethesda, Md.) · 2015Article
- TALEN-mediated apc mutation in Xenopus tropicalis phenocopies familial adenomatous polyposis.Oncoscience · 2015Article
- Inbreeding Ratio and Genetic Relationships among Strains of the Western Clawed Frog, Xenopus tropicalis.PloS one · 2015Article
- The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos.PloS one · 2015Article
- TAL effectors: tools for DNA targeting.Briefings in functional genomics · 2014Article
- TALENs mediate efficient and heritable mutation of endogenous genes in the marine annelid Platynereis dumerilii.Genetics · 2014Article
- Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease.Development (Cambridge, England) · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the past decade, Xenopus tropicalis has emerged as a powerful new amphibian genetic model system, which offers all of the experimental advantages of its larger cousin, Xenopus laevis. Here we investigated the efficiency of transcription activator-like effector nucleases (TALENs) for generating targeted mutations in endogenous genes in X. tropicalis. For our analysis we targeted the tyrosinase (oculocutaneous albinism IA) (tyr) gene, which is required for the production of skin pigments, such as melanin. We injected mRNA encoding TALENs targeting the first exon of the tyr gene into two-cell-stage embryos. Surprisingly, we found that over 90% of the founder animals developed either partial or full albinism, suggesting that the TALENs induced bi-allelic mutations in the tyr gene at very high frequency in the F0 animals. Furthermore, mutations tyr gene were efficiently transmitted into the F1 progeny, as evidenced by the generation of albino offspring. These findings have far reaching implications in our quest to develop efficient reverse genetic approaches in this emerging amphibian model.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.