ArticleThe Journal of pharmacology and experimental therapeutics2013
Ethanol activation of protein kinase A regulates GABAA α1 receptor function and trafficking in cultured cerebral cortical neurons.
Article in The Journal of pharmacology and experimental therapeutics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 22 citations in OpenAlex.
- Acute Ethanol Exposure Inhibits GABA Uptake in Embryonic Chicken Retina.Neurochemical research · 2026Article
- Review
- Regulation of GABAMembranes · 2021Review
- Endogenous Neurosteroid (3α,5α)3-Hydroxypregnan-20-one Inhibits Toll-like-4 Receptor Activation and Pro-inflammatory Signaling in Macrophages and Brain.Scientific reports · 2019Article
- Histone deacetylases mediate GABANeuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018Article
- Maternal care-related differences in males and females rats' sensitivity to ethanol and the associations between the GABAergic system and steroids in males.Developmental psychobiology · 2018Article
- Ethanol Exposure RegulatesThe Journal of pharmacology and experimental therapeutics · 2017Article
- Strain-specific programming of prenatal ethanol exposure across generations.Alcohol (Fayetteville, N.Y.) · 2017Article
- Differential regulation of synaptic and extrasynaptic α4 GABA(A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons.Neuropharmacology · 2016Article
- Ethanol Regulation of Synaptic GABAA α4 Receptors Is Prevented by Protein Kinase A Activation.The Journal of pharmacology and experimental therapeutics · 2016Article
- Regulation of Extrasynaptic GABAA α4 Receptors by Ethanol-Induced Protein Kinase A, but Not Protein Kinase C Activation in Cultured Rat Cerebral Cortical Neurons.The Journal of pharmacology and experimental therapeutics · 2016Article
- Ethanol Activation of PKA Mediates Single-Minded 2 Expression in Neuronal Cells.Molecular neurobiology · 2015Article
- Time-Dependent Effects of Ethanol on BK Channel Expression and Trafficking in Hippocampal Neurons.Alcoholism, clinical and experimental research · 2015Article
- Adolescent and adult rat cortical protein kinase A display divergent responses to acute ethanol exposure.Alcohol (Fayetteville, N.Y.) · 2014Article
- Plasticity of GABA(A) receptor-mediated neurotransmission in the nucleus accumbens of alcohol-dependent rats.Journal of neurophysiology · 2014Article
- Ethanol-induced plasticity of GABAA receptors in the basolateral amygdala.Neurochemical research · 2014Article
- Altered GABAA receptor expression and seizure threshold following acute ethanol challenge in mice lacking the RIIβ subunit of PKA.Neurochemical research · 2014Article
- Molecular and behavioral characterization of adolescent protein kinase C following high dose ethanol exposure.Psychopharmacology · 2014Article
- The neurobiology of alcohol consumption and alcoholism: an integrative history.Pharmacology, biochemistry, and behavior · 2013Review
- Whole brain and brain regional coexpression network interactions associated with predisposition to alcohol consumption.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Ethanol exposure produces alterations in GABAergic signaling that are associated with dependence and withdrawal. Previously, we demonstrated that ethanol-induced protein kinase C (PKC) γ signaling selectively contributes to changes in GABAA α1 synaptic receptor activity and surface expression. Here, we demonstrate that protein kinase A (PKA) exerts opposing effects on GABAA receptor adaptations during brief ethanol exposure. Cerebral cortical neurons from day 0-1 rat pups were tested after 18 days in culture. Receptor trafficking was assessed by Western blot analysis, and functional changes were measured using whole-cell patch-clamp recordings of evoked and miniature inhibitory postsynaptic current (mIPSC) responses. One-hour ethanol exposure increased membrane-associated PKC and PKA, but steady-state GABAA α1 subunit levels were maintained. Activation of PKA by Sp-adenosine 3',5'-cyclic monophosphothioate triethylamine alone increased GABAA α1 subunit surface expression and zolpidem potentiation of GABA responses, whereas coexposure of ethanol with the PKA inhibitor Rp-adenosine 3',5'-cyclic monophosphothioate triethylamine decreased α1 subunit expression and zolpidem responses. Exposure to the PKC inhibitor calphostin-C with ethanol mimicked the effect of direct PKA activation. The effects of PKA modulation on mIPSC decay τ were consistent with its effects on GABA currents evoked in the presence of zolpidem. Overall, the results suggest that PKA acts in opposition to PKC on α1-containing GABAA receptors, mediating the GABAergic effects of ethanol exposure, and may provide an important target for the treatment of alcohol dependence/withdrawal.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.