ArticleIntegrative biology : quantitative biosciences from nano to macro2013
Sorting single satellite cells from individual myofibers reveals heterogeneity in cell-surface markers and myogenic capacity.
Article in Integrative biology : quantitative biosciences from nano to macro, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Isolation and Expansion of Muscle Satellite Cells Using the Ice-Cold Treatment Method.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Single-cell Technology in Stem Cell Research.Current stem cell research & therapy · 2025Review
- Control of satellite cell function in muscle regeneration and its disruption in ageing.Nature reviews. Molecular cell biology · 2022Review
- The chemokine receptor CXCR4 regulates satellite cell activation, early expansion, and self-renewal, in response to skeletal muscle injury.Frontiers in cell and developmental biology · 2022Article
- A Simple Method for the Isolation andBio-protocol · 2021Article
- Sympathetic activity is correlated with satellite cell aging and myogenesis via β2-adrenoceptor.Stem cell research & therapy · 2021Article
- A novel approach for the isolation and long-term expansion of pure satellite cells based on ice-cold treatment.Skeletal muscle · 2021Article
- Microfluidics as efficient technology for the isolation and characterization of stem cells.EXCLI journal · 2021Review
- Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation.Skeletal muscle · 2020Review
- Article
- Label-Free, High-Throughput Purification of Satellite Cells Using Microfluidic Inertial Separation.Tissue engineering. Part C, Methods · 2018Article
- Article
- Article
- Established cell surface markers efficiently isolate highly overlapping populations of skeletal muscle satellite cells by fluorescence-activated cell sorting.Skeletal muscle · 2016Article
- A Microfluidic Method for the Selection of Undifferentiated Human Embryonic Stem Cells andMicrofluidics and nanofluidics · 2015Article
- Node-pore sensing enables label-free surface-marker profiling of single cells.Analytical chemistry · 2015Article
- Role of Inflammation in Muscle Homeostasis and Myogenesis.Mediators of inflammation · 2015Review
- [Cell-based therapy to treat stress urinary incontinence: which cell type at what cost?].Der Urologe. Ausg. A · 2014Article
- Towards a Treatment of Stress Urinary Incontinence: Application of Mesenchymal Stromal Cells for Regeneration of the Sphincter Muscle.Journal of clinical medicine · 2014Review
- Cell-based therapy for the deficient urinary sphincter.Current urology reports · 2013Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Traditional cell-screening techniques such as FACS and MACS are better suited for large numbers of cells isolated from bulk tissue and cannot easily screen stem or progenitor cells from minute populations found in their physiological niches. Furthermore, these techniques rely upon irreversible antibody binding, potentially altering cell properties, including gene expression and regenerative capacity. To address these challenges, we have developed a novel, label-free stem-cell analysis and sorting platform capable of quantifying cell-surface marker expression of single functional organ stem cells directly isolated from their micro-anatomical niche. Using our unique platform, we have discovered a remarkable heterogeneity in both the regenerative capacity and expression of CXCR4, β1-integrin, Sca-1, M-cadherin, Syndecan-4, and Notch-1 in freshly isolated muscle stem (satellite) cells residing on different, single myofibers and have identified a small population of Sca-1(+)/Myf5(+) myogenic satellite cells. Our results demonstrate the utility of our single-cell platform for uncovering and functionally characterizing stem-cell heterogeneity in the organ microniche.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.