ArticleNature methods2013
Quantitative genetic-interaction mapping in mammalian cells.
Article in Nature methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
67 citing papers in PubMed, 112 citations in OpenAlex.
- Quantitative analysis of genetic interactions in human cells from genome-wide CRISPR-Cas9 screens.bioRxiv : the preprint server for biology · 2026Article
- OverlappING and DivergING roles of ING4 and ING5 in cancer, differentiation and development.Frontiers in cell and developmental biology · 2026Review
- CRISPRi-TnSeq maps genome-wide interactions between essential and non-essential genes in bacteria.Nature microbiology · 2024Article
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- Integrative structure determination of histones H3 and H4 using genetic interactions.The FEBS journal · 2023Article
- Regulation of the COPII secretory machinery via focal adhesions and extracellular matrix signaling.The Journal of cell biology · 2022Article
- From systems to structure - using genetic data to model protein structures.Nature reviews. Genetics · 2022Review
- RNAi Screening Uncovers a Synthetic Sick Interaction between CtIP and the BARD1 Tumor Suppressor.Cells · 2022Article
- A new era in functional genomics screens.Nature reviews. Genetics · 2022Review
- A low-cost self-dispersing method of droplet array generation enabled by a simple reusable mask for bioanalysis and bioassays.Analytical and bioanalytical chemistry · 2022Article
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- Mass spectrometry-based protein-protein interaction networks for the study of human diseases.Molecular systems biology · 2021Review
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- Integration of multiple biological contexts reveals principles of synthetic lethality that affect reproducibility.Nature communications · 2020Article
- A Quantitative Genetic Interaction Map of HIV Infection.Molecular cell · 2020Article
- A High-Content Screen Identifies MicroRNAs That Regulate Liver Repopulation After Injury in Mice.Gastroenterology · 2020Article
- GEMINI: a variational Bayesian approach to identify genetic interactions from combinatorial CRISPR screens.Genome biology · 2019Article
- From observing to predicting single-cell structure and function with high-throughput/high-content microscopy.Essays in biochemistry · 2019Review
- In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens.Nature methods · 2019Article
- The ability of transcription factors to differentially regulate gene expression is a crucial component of the mechanism underlying inversion, a frequently observed genetic interaction pattern.PLoS computational biology · 2019Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Mapping genetic interactions (GIs) by simultaneously perturbing pairs of genes is a powerful tool for understanding complex biological phenomena. Here we describe an experimental platform for generating quantitative GI maps in mammalian cells using a combinatorial RNA interference strategy. We performed ∼11,000 pairwise knockdowns in mouse fibroblasts, focusing on 130 factors involved in chromatin regulation to create a GI map. Comparison of the GI and protein-protein interaction (PPI) data revealed that pairs of genes exhibiting positive GIs and/or similar genetic profiles were predictive of the corresponding proteins being physically associated. The mammalian GI map identified pathways and complexes but also resolved functionally distinct submodules within larger protein complexes. By integrating GI and PPI data, we created a functional map of chromatin complexes in mouse fibroblasts, revealing that the PAF complex is a central player in the mammalian chromatin landscape.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.