ArticleThe Journal of investigative dermatology2013
Remodeling of three-dimensional organization of the nucleus during terminal keratinocyte differentiation in the epidermis.
Article in The Journal of investigative dermatology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 72 citations in OpenAlex.
- KSTITCH links cellular morphology and gene expression in spatial transcriptomics.bioRxiv : the preprint server for biology · 2026Article
- Combining advanced 3D spheroid-based skin models with deep-learning-based image analysis enables in-depth investigation of keratinocyte differentiation and barrier function.Frontiers in bioengineering and biotechnology · 2026Article
- 3D-FISH Analysis of the Spatial Genome Organization in Mammalian Tissue In Situ.Methods in molecular biology (Clifton, N.J.) · 2026Article
- ESPRESSO: spatiotemporal omics based on organelle phenotyping.Nature methods · 2025Article
- Molecular and mechanical signatures contributing to mouse epidermal differentiation and barrier formation.eLife · 2025Article
- Review
- Regulation of chromatin modifications through coordination of nucleus size and epithelial cell morphology heterogeneity.Communications biology · 2025Article
- Epigenetic Regulatory Processes Involved in the Establishment and Maintenance of Skin Homeostasis-The Role of Microbiota.International journal of molecular sciences · 2025Review
- Dynamics and Epigenetics of the Epidermal Differentiation Complex.Epigenomes · 2024Review
- Review
- Chromatin Landscape Governing Murine Epidermal Differentiation.The Journal of investigative dermatology · 2023Article
- Deciphering a Message from the Nucleus: How Transcription Factors and Spatial Chromatin Interactions Orchestrate Epidermal Differentiation.The Journal of investigative dermatology · 2023Article
- Article
- Spatially Guided Construction of Multilayered Epidermal Models Recapturing Structural Hierarchy and Cell-Cell Junctions.Small science · 2022Article
- Extracellular Adhesive Cues Physically Define Nucleolar Structure and Function.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Nuclear envelope, chromatin organizers, histones, and DNA: The many achilles heels exploited across cancers.Frontiers in cell and developmental biology · 2022Review
- Article
- Culturing Keratinocytes on Biomimetic Substrates Facilitates Improved Epidermal Assembly In Vitro.Cells · 2021Article
- Article
- Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The nucleus of epidermal keratinocytes (KCs) is a complex and highly compartmentalized organelle, whose structure is markedly changed during terminal differentiation and transition of the genome from a transcriptionally active state seen in the basal and spinous epidermal cells to a fully inactive state in the keratinized cells of the cornified layer. Here, using multicolor confocal microscopy, followed by computational image analysis and mathematical modeling, we demonstrate that in normal mouse footpad epidermis, transition of KCs from basal epidermal layer to the granular layer is accompanied by marked differences in nuclear architecture and microenvironment including the following: (i) decrease in the nuclear volume; (ii) decrease in expression of the markers of transcriptionally active chromatin; (iii) internalization and decrease in the number of nucleoli; (iv) increase in the number of pericentromeric heterochromatic clusters; and (v) increase in the frequency of associations between the pericentromeric clusters, chromosomal territory 3, and nucleoli. These data suggest a role for nucleoli and pericentromeric heterochromatin clusters as organizers of nuclear microenvironment required for proper execution of gene expression programs in differentiating KCs, and provide important background information for further analyses of alterations in the topological genome organization seen in pathological skin conditions, including disorders of epidermal differentiation and epidermal tumors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.